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Figure 2 | BMC Biochemistry

Figure 2

From: Redox biology of Mycobacterium tuberculosis H37Rv: protein-protein interaction between GlgB and WhiB1 involves exchange of thiol-disulfide

Figure 2

WhiB1 physically interacts with GlgB in vitro. GST pull down assay to show GlgB interacts with WhiB1. WhiB1-GST/WhiB4-GST/GST-alone were co-expressed with GlgB-6× His in E. coli BL21(DE3). Interaction was carried out using glutathione agarose beads as described in 'materials and methods'. The left panel is 10% SDS-PAGE stained with silver nitrate, whereas the right panel is an immuno-blot showing the co-elution of GlgB along with WhiB1-GST. The asterisks denote the position of WhiB4-GST (*), WhiB1-GST (**) and GST-alone (***).

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BMC Biochemistry

ISSN: 1471-2091

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