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Figure 1 | BMC Biochemistry

Figure 1

From: Redox biology of Mycobacterium tuberculosis H37Rv: protein-protein interaction between GlgB and WhiB1 involves exchange of thiol-disulfide

Figure 1

Yeast two-hybrid analysis using whiB 1 as 'bait'. Saccharomyces cerevisiae cells were transformed with plasmids: pDBD-B1 (bait), pADMtb (prey) and pSH18-34 (reporter plasmid) and the transformants were selected on Glu+His-Ura-Trp- plates. The transformants were pooled and allowed to grow in a liquid medium supplemented with galactose (1%) for 6 h at 30°C in order to induce the expression of gene cloned in pADMtb. After induction, the cells were plated on Gal+His-Ura-Trp- Leu- plates. Viable clones were further tested for specific interaction by dilution plating on Gal+His-Ura-Trp-Leu- and Glu+His-Ura-Trp-Leu- plates. Growth of two representative positive clones in dilution plating is shown in the picture. Figures on the top indicate the total number of cells spotted on the respective plate for dilution plating.

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