Figure 2

Differential staining for the AAA activities on ChEs from eel AChE and human serum BChE. Electrophoresis was performed on 3.5% native polyacrylamide gel slabs under non-denaturing conditions as detailed in 'Materials and methods'. All lanes were loaded with 1 U each of AAA on eel AChE and AAA on human serum BChE . After electrophoresis, 'A' was incubated in substrate solution without any inhibitor; 'B' was incubated in substrate solution containing 100 μM of iso-OMPA; and 'C' was incubated in substrate solution containing 100 μM of BW 284c51. After incubation for 40 min, the gels were stained for the AAA activity. The upper and lower arrows denote the AAA activity due to BChE and AChE, respectively.