Fig. 2From: Identification of the major diacylglycerol acyltransferase mRNA in mouse adipocytes and macrophagesTTP and DGAT mRNAs were stable in mouse adipocytes. The differentiated 3 T3-L1 adipocytes were serum-starved for 4 h in DMEM without any supplementation and collected at various time points (0, 30, 60, and 90 min after 4-h starvation) for RNA extraction and cDNA synthesis. TaqMan qPCR assay evaluated Rpl32, Ttp, Dgat1, and Dgat2 gene expression. The data represent the mean and standard deviation of four assaysBack to article page