Fig. 3
Unbundling (dissociation) of histatin-3 or −5 bundled actin filaments by NaCl, DNase1 and cofilin. a Effect of 0–200 mM NaCl on the extent of bundling of 4 μM F-actin bundled by 16 μM histatin-3 or histatin-5 was studied by low speed centrifugation as described in Methods. Upper panel, quantitative evaluation of bundling, lower panel, representative SDS-PAGE of the supernatants. b and c Unbundling kinetics by 9 μM DNase1, 9 μM cofilin and 200 mM NaCl of 4 μM F-actin bundled by b 8 μM histatin-3 or c 16 μM histatin-5. Unbundling was followed by light scattering change as described in Methods