a Cation | b Base pair at the 3′ end | c V max | d K m | V max / K m | e Standard extension efficiency, f ext | f Relative fidelity | g P-value |
---|---|---|---|---|---|---|---|
%/min | μM | ||||||
hMg2+ | C.G | 21.4 ± 2.0 | 0.37 ± 0.13 | 57.8 ± 19.2 | 1 |  |  |
G.C | 39.9 ± 14.5 | 0.27 ± 0.13 | 147.8 ± 40.6 | 1 | |||
C.T | 22.5 ± 2.1 | 396 ± 28 | 5.7 (±0.9) x 10−2 | 9.9 (±1.7) x 10−4 |  |  | |
C.A | 11.1 ± 2.9 | 445 ± 188 | 2.5 (±0. 57) x 10−2 | 4.3 (±0.94) x 10−4 | |||
C.C | 0.70 ± 0.29 | 77 ± 14 | 9.1 (±2.2) x 10−3 | 1.6 (±0.38) x 10−4 | |||
G.T | 18.6 ± 3.3 | 157 ± 44 | 0.12 ± 0.03 | 8.1 (±2.5) x10−4 | |||
G.A | 0.95 ± 0.04 | 196 ± 50 | 4.8 (±1.6) x 10−3 | 3.2 (±1.0) x 10−5 |  | ||
Zn2+ | C.G | 0.62 ± 0.23 | 0.04 ± 0.01 | 15.5 ± 5.8 | 1 | 1 |  |
G.C | 0.39 ± 0.10 | 0.04 ± 0.01 | 9.8 ± 1.9 | 1 | 1 | ||
C.T | 2.9 ± 1.0 | 591 ± 63 | 4.9 (±2. 3) x 10−3 | 3.2 (±1.6) x 10−4 | 3.1 | 0.026 | |
C.A | 3.3 ± 1.0 | 1240 ± 186 | 2.7 (±1.3) x 10−3 | 1.7 (±0.75) x 10−4 | 2.5 | 0.029 | |
C.C | 0.50 ± 0.14 | 914 ± 457 | 5.4 (±4.6) x 10−4 | 3.5 (±3.4) x 10−5 | 4.6 | 0.023 | |
G.T | 6.8 ± 1.8 | 1223 ± 568 | 5.6 (±2.8) x 10−3 | 5.7 (±3.2) x 10−4 | 1.4 | 0.375 | |
G.A | N.D. |