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Figure 5 | BMC Biochemistry

Figure 5

From: Histone deacetylase inhibitor sodium butyrate suppresses DNA double strand break repair induced by etoposide more effectively in MCF-7 cells than in HEK293 cells

Figure 5

Sodium butyrate modulates the nuclear expression of DSB repair proteins induced by etoposide in MCF-7 and MEL293 cells. HEK293 and MCF-7 cells were pre-treated with DMSO vehicle or 0.5, 2.0, 8.0 mM sodium butyrate for 4 h before exposure to DMSO vehicle or 10 μM etoposide for 20 h. After the treatment period, the cells were harvested, and then soluble nuclear protein and chromatin bound protein were extracted using a subcellular protein fraction kit. 50 μg proteins were loaded for western blot analysis. Coomassie blue staining gel is shown as loading control. Results are representative of three independent experiments.

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BMC Biochemistry

ISSN: 1471-2091

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