Skip to main content
Figure 4 | BMC Biochemistry

Figure 4

From: Histone deacetylase inhibitor sodium butyrate suppresses DNA double strand break repair induced by etoposide more effectively in MCF-7 cells than in HEK293 cells

Figure 4

Sodium butyrate statistically increases γ-H2AX foci induced by etoposide in MCF-7 cells but not HEK293 cells. HEK293 and MCF-7 cells growing on slides in 24 well plates were exposed to DMSO vehicle or 2.0 mM sodium butyrate for 4 h followed by 20 h DMSO vehicle or 10 μM etoposide treatment. Following the treatment period, cells were fixed and assessed for γ-H2AX foci. (A) The average numbers of γ-H2AX foci per cell are shown. The inset shows a representative image of staining for a γ-H2AX-positive cell. (B) The % of foci-containing cells with >5 foci was calculated. *P< 0.05 and **P< 0.01. Results represent the mean +SD of 6 samples with 100 to 300 cells counted per slide.

Back to article page

BMC Biochemistry

ISSN: 1471-2091

Contact us