Figure 1
Sodium butyrate suppresses the proliferation of HEK293 and MCF-7 cells to different extents. (A) HEK293 cells were incubated with DMSO vehicle, 0.1, 0.5, 2.0, or 8.0 mM sodium butyrate for the indicated times. After the treatment period, cells were assessed for cell proliferation by CCK-8 assay. The A450nm value corresponds to the amount of cells. *P< 0.05 and **P< 0.01 versus the corresponding time for vehicle-treated cells. (B) MCF-7 cells were incubated with DMSO vehicle or sodium butyrate and were assessed by CCK-8 assay as in panel A. (C) MCF-7 cell growth inhibition was compared for HEK293 versus MCF-7 cells after treatment with 0.5 or 4.0 mM sodium butyrate for the indicated times. Results represent the CCK-8 assay values at each respective drug treatment relative to that of the DMSO vehicle control. *P< 0.05 and **P< 0.01 for MCF-7 cells versus the corresponding treatment for HEK293 cells. All data represent the means +/− SD of 3 experiments performed in triplicate.