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Table 3 Kinetic parameters for free AMPD before and after removal of low molecular weight ions and metabolites by centrifugation through Sephadex G50 columns

From: Regulation of 5'-adenosine monophosphate deaminase in the freeze tolerant wood frog, Rana sylvatica

 

Before spun columns

After spun columns

 

S0.5 AMP (mM)

n H

S0.5 AMP (mM)

n H

Control

2.16 ± 0.11

1.76 ± 0.06

1.35 ± 0.08a

2.37 ± 0.17 a

Frozen

2.19 ± 0.15

2.76 ± 0.10 a

1.68 ± 0.21 a

2.76 ± 0.27

  1. Data are means ± SEM, for determinations on muscle preparations from n = 4 individual frogs. a – Significantly different from the corresponding free control value prior to desalting by the Student's t-test, P < 0.05.
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BMC Biochemistry

ISSN: 1471-2091

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