Figure 2
From: Role of the linker region in the expression of Rhizopus oryzae glucoamylase
Expression of recombinant Ro GAs in Saccharomyces. (A) Starch plate assay for GA expression in 10 μL (A600 = 2) S. cerevisiae cells carrying pS1 (lane 1), pS1/full-length GA (lane 2) and pS1/GAΔ132–167 (lane 3) plasmids growth on a SD plate containing 0.5% soluble starch at 30°C and stained with 0.01% iodine solution. (B) Yeast strain was transformed with expression plasmids containing the full-length or mutant GA insert. After incubation at 30°C for 3 days, the supernatants were collected and concentrated. Culture supernatant (10 μL) from each clone was examined by Western blotting using anti-GA antibody. Upper panel, lane 1, vector only; lane 2, full-length GA; lane 3, GAΔ168–604 and lane 4, GAΔ132–604; lower panel, lane 1, vector only; lane 2, full-length GA; lane 3, GAΔ132–167; lane 4, GAΔ26–131; lane 5, GAΔ26–145; lane 6, GAΔ26–160 and lane 7, GAΔ26–167.