Figure 5
Normalized amplitudes of SPR sensorgrams obtained by injection of recoverin forms into flow cells coated with PC:PE:cholesterol. Injection of WT recoverin (black bars) and Rc2–190 (gray bars) into the flow cell at the indicated concentrations. The immobilized lipid surface contained a mixture of PC:PE:cholesterol (25:25:50). The binding ratios were 1.45 (7 μM), 1.1 (18 μM), 1.28 (30 μM) and 1.14 (μM). Data shown were collected from 2 experiments and are representative for a different set of experiments with cholesterol containing lipids. The running buffer containing 0.2 mM CaCl2 in all cases.