Figure 2
Binding of myristoylated recoverin to lipid mixtures with different content of PS. Binding of WT recoverin (filled circles) and Rc2–190 (open circles) to lipid mixtures was analyzed by an equilibrium centrifugation assay as described in the Methods section. The content of PS was adjusted as indicated below. 0% PS: 50% (w/w) phosphatidylethanolamine, 50% (w/w) phosphatidylcholine; 10% PS: 45% (w/w) phosphatidylethanolamine, 45% (w/w) phosphatidylcholine; 20% PS: 40% (w/w) phosphatidylethanolamine, 40% (w/w) phosphatidylcholine; 30% PS, 35% (w/w) phosphatidylethanolamine, 35% (w/w) phosphatidylcholine; 40% PS: 30% (w/w) phosphatidylethanolamine, 30% (w/w) phosphatidylcholine; 50% PS: 25% (w/w) phosphatidylethanolamine, 25% (w/w) phosphatidylcholine; 60% PS: 20% (w/w) phosphatidylethanolamine, 20% (w/w) phosphatidylcholine; 80% PS: 10% (w/w) phosphatidylethanolamine, 10% (w/w) phosphatidylcholine; 100% PS. Ratios of bound WT to bound Rc2–190 were 1.5 (0% PS), 2.0 (30% PS), 2.7 (60% PS) and 1.4 (100% PS).