Skip to main content

Table 4 Use of DCCD in release rebinding experiments involving rat brain HK and rat, bovine and human brain mitochondria

From: A study on the two binding sites of hexokinase on brain mitochondria

Steps

1

2

3

4

Process

%Release (with G6P)

Blocking (with DCCD)

%Release (with KSCN)

Rebinding

Rat

81 ± 3

Yes

30 ± 2.1

N.D.

Bovine

38 ± 2.7

Yes

19 ± 1.7

N.D.

Human

48.5 ± 3.5

Yes

17.5 ± 2.5

N.D.

  1. Mitochondria from three species were subjected to HK release by G6P (2 mM) in the first step. Then, the exposed binding sites of G6P – treated mitochondria were blocked by DCCD (0.01 mM). At the third step, the remaining HK activity on this DCCD-treated mitochondria was released with 45 mM KSCN. Finally the depleted (both sites) blocked (G6P-sensitive sites) mitochondria were used for the rebinding process, according to details described under Methods. N.D.; not detectable
Back to article page

BMC Biochemistry

ISSN: 1471-2091

Contact us