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Figure 2 | BMC Biochemistry

Figure 2

From: Acyl-CoA-binding and self-associating properties of a recombinant 13.3 kDa N-terminal fragment of diacylglycerol acyltransferase-1 from oilseed rape

Figure 2

Binding of acyl-CoAs to BnDGAT1(1–116)His6. Panels A and B: Binding of oleoyl-CoA or erucoyl-CoA to BnDGAT1(1–116)His6. Lipidex-1000 binding assays were conducted in duplicate in 10 mM potassium phosphate buffer, pH 7.4, at 30°C with [1-14C]acyl-CoAs and 0.2 μM protein. Incubations were set up without protein to correct for acyl-CoA that was not adsorbed by the Lipidex-1000. Data points represent mean values. Panels C and D: Scatchard plot [26] analysis of the binding of 18:1-CoA or 22:1-CoA to BnDGAT1(1–116)His6.

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BMC Biochemistry

ISSN: 1471-2091

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