Figure 4

Mapping of DX136 and OX108 epitopes on the N-terminal domain of human CD200R. (A). The mutants giving complete or nearly complete inhibition of CD200 binding, determined by BIAcore analysis, are indicated with red circles (<35% binding compared to non-mutated WT hCD200R protein), whereas those giving partial effects (35–70% binding) are depicted in orange. Data from [33].(B). The mutants giving severe inhibition (<35% compared to WT) of OX108 and DX136 mAb binding, as determined by microarray analysis, are depicted in blue and yellow respectively. Dark green circles represent mutants that severely impaired both OX108 and DX136 mAb binding. Mutants partially affecting DX136 binding (35–50%) are shown in pale yellow. Mutants that severely affect DX136 binding (35% binding or less) but have only a partial effect on OX108 binding (50%) are represented in pale green. Open circles depict mutants that did not affect the binding of CD200, OX108 or DX136 mAb. The CD200R model is based on a typical Ig V domain from the human junctional adhesion molecule-1 (JAM1) [34]. The beta sheets are labelled with the GFC face orientated in front and the BED face behind.