Figure 3

The atypical Kazal domain EPI1a inhibits subtilisin A. The remaining protease activity of subtilisin A was measured after incubating with rEPI1a, rEPI1b or without protease inhibitors (Std) using the QuantiCleave™ Protease Assay Kit as described in the methods. Activity is expressed as a percentage of total protease activity in the absence of protease inhibitors. The bars correspond to the mean of three independent experiments with three replications for each experiment. The error bars represent the standard errors calculated from the mean of three experiments.