Integrated allosteric regulation in the S. cerevisiae carbamylphosphate synthetase – aspartate transcarbamylase multifunctional protein
© Serre et al; licensee BioMed Central Ltd. 2004
Received: 19 December 2003
Accepted: 05 May 2004
Published: 05 May 2004
The S. cerevisiae carbamylphosphate synthetase – aspartate transcarbamylase multifunctional protein catalyses the first two reactions of the pyrimidine pathway. In this organism, these two reactions are feedback inhibited by the end product UTP. In the present work, the mechanisms of these integrated inhibitions were studied.
The results obtained show that the inhibition is competitive in the case of carbamylphosphate synthetase and non-competitive in the case of aspartate transcarbamylase. They also identify the substrate whose binding is altered by this nucleotide and the step of the carbamylphosphate synthetase reaction which is inhibited. Furthermore, the structure of the domains catalyzing these two reactions were modelled in order to localize the mutations which, specifically, alter the aspartate transcarbamylase sensitivity to the feedback inhibitor UTP. Taken together, the results make it possible to propose a model for the integrated regulation of the two activities of the complex. UTP binds to a regulatory site located in the vicinity of the carbamylphosphate synthetase catalytic subsite which catalyzes the third step of this enzyme reaction. Through a local conformational change, this binding decreases, competitively, the affinity of this site for the substrate ATP. At the same time, through a long distance signal transmission process it allosterically decreases the affinity of the aspartate transcarbamylase catalytic site for the substrate aspartate.
This investigation provides informations about the mechanisms of allosteric inhibition of the two activities of the CPSase-ATCase complex. Although many allosteric monofunctional enzymes were studied, this is the first report on integrated allosteric regulation in a multifunctional protein. The positions of the point mutations which specifically abolish the sensitivity of aspartate transcarbamylase to UTP define an interface between the carbamylphosphate synthetase and aspartate transcarbamylase domains, through which the allosteric signal for the regulation of aspartate transcarbamylase must be propagated.
Although numerous allosteric enzymes were studied, much less information is available concerning the coordinated regulation of activities in multienzymatic complexes. Two feedback inhibited multienzyme complexes were studied in Saccharomyces cerevisiae, the N-acetylglutamate synthase/N-acetyl glutamate kinase  and the carbamylphosphate synthetase – aspartate transcarbamylase  complexes.
- The glutaminase (GLNase) domain which hydrolyzes glutamine and transfers ammonia to the carbamylphosphate synthetase domain .
- The CPSase domain which catalyzes the synthesis of carbamylphosphate from two molecules of ATP, bicarbonate and ammonia in a stepwise fashion that involves three partial reactions: the activation of bicarbonate by ATP, the reaction of the activated species, carboxyphosphate, with ammonia to form carbamate and the ATP-dependent phosphorylation of carbamate to form carbamylphosphate [3, 14]:
(1) ATP-Mg + HCO32- -OCOOPO32- + ADP-Mg
(2) -OCOOPO32- + NH3 (Gln) NH2COO- + Pi + (Glu)
(3) NH2COO- + ATP-Mg NH2COOPO32- + ADP-Mg
- The inactive pDHO domain.
- The ATCase domain which catalyzes the reaction of carbamylphosphate and aspartate to form carbamylaspartate.
The N- and C-halves of CPSases from all organisms examined so far, show a significant degree of sequence similarity [10, 15–20], an observation which was interpreted to mean that the genes coding for these enzymes evolved through a process of gene duplication, fusion, and differentiation [15, 16]. The two domains corresponding to these two halves are called CPS-A and CPS-B. Unexpectedly, it was discovered that each of these two domains of the mammalian CAD CPSase are able to independently catalyze the formation of carbamylphosphate provided that they dimerize [21, 22]. In the same way, a truncated yeast bifunctional protein lacking the GLNase and CPS-A domains (CBApD) was shown to possess the CPSase activity regulated by UTP . In contrast, the ATCase domain was no longer sensitive to this nucleotide, indicating that the two catalytic activities are controlled by distinct mechanisms .
In order to identify amino acid residues implicated in the feedback inhibition by UTP, genetics were used to positively select in vivo and characterize missense mutations in the URA2 gene, which specifically affect the feedback – inhibition of ATCase . In these mutants ATCase is no longer inhibited by UTP although CPSase retains full sensitivity to this effector, indicating again that UTP affects the activities of CPSase and ATCase by different mechanisms .
In the present work the use of S. cerevisiae mutants in which single amino acid replacements abolish the sensitivity of ATCase to UTP allowed to study specifically the process of feedback inhibition of the CPSase domain by this nucleotide. In addition, the reaction step affected by the feedback inhibitor UTP was identified in both the entire complex and in the truncated protein (CBApD). Moreover a computational approach was used to predict the structures of the CPSase and ATCase domains. The results obtained provide informations about the integrated allosteric regulation of the two enzymatic activities of the complex, and indicate that the regulatory site is located in the CPS-B domain. The modelling defines an interface between the CPSase and ATCase domains for the transmission of the allosteric signal.
Inhibition of the CPSase and ATCase activities of the complex
UTP inhibition of the coupled reaction
UTP inhibition of ATCase
UTP inhibition of CPSase
Position of the missense mutations affecting the sensitivity of ATCase to UTP.
Determination of the CPSase reaction step influenced by the feedback inhibitor UTP
Wild type complex
Modelling of the CPSase and ATCase domains and localization of the missense mutations affecting the sensitivity of ATCase to UTP
Amino acid sequences alignment
Modelling of the S. cerevisiae CPSase and ATCase domains
Localization of the ATCase desensitizing mutations
In the S. cerevisiae CPSase-ATCase complex the two activities are feedback inhibited by the end-product UTP [12, 13]. In the case of CPSase, the results reported here indicate that the substrate whose binding is altered by this effector is ATP as shown previously in the case of E. coli CPSase . In the case of yeast ATCase, UTP decreases the affinity for the substrate aspartate . Taken together with previously published observations, the results reported here show that the feedback inhibition of the yeast complex is of absolute competitive nature in the case of CPSase and of partial competitive nature in the case of ATCase. This last behavior is characteristic of a process of allosteric inhibition in which the effector binds to a regulatory site distinct from the catalytic site. In the case of CPSase the absolute competition indicates that UTP binds close enough to directly prevent the binding of ATP to this site through a local conformational change.
Several lines of evidence show that the regulatory site where UMP (procaryote CPSases) or UTP (enkaryote CPSases) binds is localized in the B3 subdomain (Fig. 1) [27, 28, 35]. Among the two steps of the CPSase reaction which, each, use a molecule of ATP (steps 1 and 3) only the third one is inhibited by UTP (Fig. 6). This partial reaction is specifically catalyzed by the B2 subdomain. Thus, it appears that the UTP binding site is located in the CPS-B domain which catalyzes the partial reaction which is specifically inhibited by this nucleotide, a feature which relates to the absolute competitive inhibition reported above. In the E. coli enzyme this regulatory site and the CPS-B catalytic site are distant by approximately 20 Å . However, in the yeast complex this distance might be lower as the result of interactions with the ATCase and pDHOase domains. Alternatively, UTP might act through a different mechanism in the yeast complex and might provoke the competitive inhibition through binding to the CPS-B ATP binding site.
The first partial reaction catalyzed by the domain A is not affected by the presence of UTP. It was shown previously that the dimer of the isolated B domain is able to catalyze the synthesis of carbamylphosphate . Interestingly, in this case, the first step of the CPSase reaction becomes sensitive to UTP (Fig. 7). Thus, the reaction normally catalyzed by the A domain becomes sensitive to UTP when it is catalyzed by the B domain which contains the allosteric UTP binding site. Taken together, these observations indicate that although it is homologous to the B3 domain, the A3 subdomain is unable to bind UTP.
As far as the feedback inhibition of the ATCase activity is concerned, several lines of evidence show that the presence of the CPSase domain of the S. cerevisiae complex is necessary for the ATCase domain to be sensitive to UTP, and that a single UTP binding site is located in the CPSase domain. Separation of the CPSase and ATCase domains by limited proteolysis  or genetic engeneering [23–25, 38] leads to the desensitization of the ATCase domain. Taken together with the results reported here concerning the partial competitive character of the ATCase feedback inhibition, the requirement of the CPSase domain strongly suggests that the CPSase and the ATCase catalytic sites are both under the influence of the UTP binding regulatory site located in the B3 subdomain.
The results obtained provide informations about the mechanisms of allosteric inhibition of the two activities of the CPSase-ATCase complex. It is of particular interest that the mutations which specifically abolish the sensitivity to UTP of the ATCase reaction, without altering the CPSase inhibition, are clustered on the surface of either the CPS-B domain or the ATCase domain. This observation strongly suggests that these two regions constitute the interface between the two domains, interface through which the regulatory signal must be transmitted from the CPSase regulatory site to the catalytic site of the ATCase domain. This transmission could involve either a specific path between this interface and the catalytic site of ATCase, or a more global conformational change of this ATCase domain leading to a decrease of the affinity of this site for aspartate.
This is an original example of integrated allosteric regulation in a multifunctional complex that the catalytic domain of one activity is the allosteric site for the other activity.
Plasmids and strains
The 14.0-kb plasmid pC4-URA2 contains the yeast ura2 gene encoding the bifunctionnal CPSase-ATCase complex . The pSV-CBApD recombinant plasmid encodes a protein CBApD, that possesses the C-terminal half of CPSase (CB), linked to the pDHO (pD) and the ATCase (A) domains .
The S. cerevisiae LJ5 strain was transformed by pC4 carrying a wild-type or mutated ura2 allele . The LJ5 recipient strain was chosen because it is devoid of endogenous CPSase and ATCase activities.
The E. coli mutant L673 strain , defective in carA and carB, as well as the Lon-protease, was a gift from Dr. Carol Lusty (Public Health Research Institute of the City of New York). The genes carA and carB encode the small and large subunits of E. coli carbamoyl phosphate synthetase, respectively. The E. coli host strain EK1104  lacks the pyrB1 genes. E. coli EK1104 and L673 cells were transformed with pC4-URA2 and pSV-CBApD respectively.
Cell growth and preparation of cell-free extracts
S. cerevisiae LJ5 strain harboring the recombinant plasmids was grown on YNB (6.7 g yeast nitrogen base / 1.2% glucose) at 30°C. Supplements were added to a 50 mg/ml final concentration. E. coli EK1104 and L673 cells harboring the recombinant plasmids were routinely grown from a single colony in 2xYT media supplemented with 100 μg/ml ampicillin. For induction of recombinant proteins under control of the pyrB1 promoter, the EK1104 and L673 cells were grown in a minimal media consisting of 6 g/l Na2HPO4, 3 g/l KH2PO4, 1 g/l NH4Cl, 5 g/l casamino acids, 4 g/l glucose, 0.5 mg/l ZnSO4.7H2O, 0.1 mM CaCl2, 1 mM MgSO4.7H2O, 10 mg/l tryptophan, supplemented with 12 μg/ml uracil and 100 μg/ml ampicillin. Under these conditions, there is sufficient uracil to sustain growth for about 19 to 21 hours, after which time, uracil is exhausted, growth is slowed, and the recombinant protein is expressed. Growth was monitored spectrophotometrically at 600 nm. The cells were harvested in late exponential phase or early stationary phase, by centrifugation at 3000 g for 30 minutes in a Centrikon T-124 centrifuge. The cells were resuspended in 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 1 mM DTT, and disrupted by sonication three times for one minute on ice, using a Biosonik III sonifier set at 20,000 kHz. The sonicate was cleared by centrigugation at 12,000 g for 30 minutes at 4°C. These extracts were dialyzed in order to eliminate all the metabolites, including nucleotides, which might interfere with enzyme assays. Protein concentrations were assayed by the Lowry method .
Enzymatic activities were tested on crude dialyzed extracts as described by Penverne & Hervé . The ATCase activity was tested as described by Denis-Duphil et al. . The standard conditions used were 30 mM (14C)aspartate (0.03 μCi/μmol), 10 mM carbamylphosphate, and 50 mM Tris-HCl, pH 7.5. The assays were conducted at 30°C for 10 minutes. The CPSase activity of the yeast CPSase-ATCase and the CBApD complexes were assayed in the presence of 5 μg of E. coli ATCase catalytic subunits to efficiently trap all the unstable carbamylphosphate formed. The standard conditions used were 50 mM Tris-Ac, pH 7.5, 100 mM KCl, 100 mM NH4Cl, 150 mM (14C)sodium bicarbonate (0.168 μCi/μmol), 20 mM magnesium acetate, 10 mM ATP, and 50 mM aspartate. The assays were conducted at 30°C for 30 minutes. The extracts samples were extensively dialyzed immediately before the enzymatic tests in order to eliminate all the metabolites (including nucleotides) potentially able to interfere with the activities.
The overall carbamylphosphate synthetase – aspartate transcarbamylase activity was tested as described by Penverne et al.  without the addition of E. coli ATCase catalytic subunits. The partial reaction 1 of CPSase was assayed at 25°C by coupling the production of MgADP to the oxydation of NADH through the inclusion of pyruvate kinase, PEP, and lactate dehydrogenase in the assay mixture. Disappearance of NADH was followed continuously by monitoring the decrease in absorbance at 340 nm with a strip chart recorder. Each cuvette contained in a final volume of 1.0 ml the following: 50 mM Tris-Ac, pH 7.5, 100 mM KCl, 20 mM MgCl2, 0.2 mM NADH, 1 mM PEP, 50 mM NaHCO3, 10 mM L-glutamine, 10 mM MgATP, 0.1 mg of pyruvate kinase, and 0.15 mg of lactate dehydrogenase. The inhibitor UTP, if present, was 10 mM. The reaction was started with the addition of 100 μl of dialyzed crude extract. The partial reaction 3 of CPSase was measured at 25°C by coupling MgATP production with the reduction of NADP with hexokinase, glucose, and glucose-6-phosphate dehydrogenase. All cuvettes contained 50 mM Tris-Ac, pH 7.5, 100 mM KCl, 20 mM MgCl2, 1 mM NADP, 10 mM glucose, 1 unit each of hexokinase and glucose-6-phosphate dehydrogenase, 6 mM of MgADP (≈ Km), and 10 mM carbamoyl phosphate. The inhibitor UTP, if present, was 10 mM. The reaction was started by the addition of 100 μl of dialyzed crude extract. It was verified that the CPSase reaction was fully dependent on the presence of the three substrates. In the case of the L673 strain these controls were already published . The same full requirement was observed in the case of the EK1104 strain. The bicarbonate ATPase-dependent reaction was undetectable in absence of bicarbonate.
Assay for UTP inhibition
The sensitivity of CPSase and ATCase to the feedback inhibitor UTP was assayed under the standard conditions described above in the presence of varying concentrations of this effector.
Sequences of E. coli CPSase (CARB, SwissProt P00968), E. coli ATCase catalytic chain (PYRB, SwissProt P00479) and S. cerevisiae CPSase-ATCase complex (PYR1, SwissProt P07259) were used. The sequences were aligned using the BIONET program FASTP (BLOSUM 50 matrix, ktup = 2) and Protein Information Ressource program ALIGN. Input parameters were chosen empirically.
Homology modelling of the S. cerevisiae CPSase and ATCase domains
The three-dimensional structure of the S. cerevisiae CPSase and ATCase domains were modelled by comparative protein modelling methods and energy minimization using the program SWISS-MODEL  in the optimized mode. The 2.10 Å coordinate set for the CPSase from E. coli  was used as the template for modelling the yeast CPSase monomer. The 2.5 Å structure of the E. coli ATCase complexed with the bisubstrate analogue N-(phosphonoacetyl)-L-aspartate  was used as the template for modelling the yeast ATCase monomer. Swiss-PdbViewer 3.5  was used to analyse and visualize the structures.
List of abbreviations
- The abbreviations used are:
CPSase, carbamylphosphate synthetase
the yeast domain that exibits sequence similarity to functional DHOases but which lacks activity
the truncated yeast complex consisting of the CPS-B domain fused to the ATCase domain via the pDHO domain
the subdomain corresponding to the amino half of the CPSase synthetase domain or subunit
- CPS-B and CB:
the subdomain corresponding to the carboxy half of the CPSase synthetase domain or subunit.
Our research was supported by the CNRS, Université Paris 6, and Université Paris 7.
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