Figure 3
From: Selective inhibition of c-Myb DNA-binding by RNA polymers
Titration of the poly(G) inhibition. NR1R2R3 (40 fmol) and R2R3 (20 fmol) were incubated separately with 20 fmol MRE-containing radiolabelled probe and increasing amounts of poly(G). The binding reactions were incubated for 15 minutes at 25°C and subsequently analyzed by EMSA and phosphorimaging. The intensities of the complex bands were quantified by phosphorimaging software and plotted as percentage of the complex band intensity when no homoribopolymers was added.