Figure 4From: Site-specific mutagenesis of Drosophila proliferating cell nuclear antigen enhances its effects on calf thymus DNA polymerase δEffect of various purified PCNA fractions on the processivity of nucleotide incorporation by calf thymus pol δ. Incorporation of [α32P]dTMP by calf thymus pol δ was monitored by standard denaturing PAGE. The substrates used were (dA)~500-(dT)12–18 as template-primer and [α-32P]dTTP. Concentrations of PCNA, both wild-type and mutant proteins, are as indicated. h, human; dr, Drosophila melanogaster. NH2-terminally his-tagged-PCNA fractions are as indicated; wt, wild-type; Q123V, recombinant Drosophila PCNA containing a single amino acid, glutamine at position 123, changed to valine; dr119-133h, recombinant Drosophila PCNA containing the entire interdomain connector loop (amino acids 119-133) replaced with the corresponding human PCNA amino acids.Back to article page