Ca2+ binding to complement-type repeat domains 5 and 6 from the low-density lipoprotein receptor-related protein

BMC Biochemistry

Table 1 Ca²⁺ binding properties of various CR-domains

*CR-domain*	Ca²⁺ *affinity*(K_D) μM	*References*	*Method* ^a)	*Conditions*
CR1^LDLR	7	[22]	Fluorescence	25°C
	10	[30]
CR2^LDLR	14	[25]
CR5^LDLR	0.040	[27]	Fluorescence	pH 7.0
	0.070	[20, 27]	Fluorescence	pH 7.0
	0.500	[6]	ITC	pH 7.4, 30°C
	13	[6]	ITC	pH 5.0, 30°C
CR6^LDLR	0.200	[27]	Fluorescence	pH 7.0
CR3^LRP	8	[6]	ITC	pH 7.4, 30°C
	24	[22]	Fluorescence	25°C
CR5^LRP	11	The present data	Rate dialysis	pH 7.4, 37°C
CR6^LRP	11	The present data	Rate dialysis	pH 7.4, 37°C
CR7^LRP	13	[6]	ITC	pH 7.4, 30°C
CR8^LRP	6	[6]	ITC	pH 7.4, 30°C
	13	[22]	Fluorescence	25°C
CR^Tva	40	[23]	ITC	pH 7.4, 30°C

^a) ITC; Isothermal titration calorimetry CRX^LDLR, CRX^LRP, and CR^Tva denote the X th CR-domain counting from the amino-terminal end of LDLR, LRP and the Tva receptor of avian leucosis and sarcoma virus type A, respectively.

ISSN: 1471-2091