Regulated interaction between polypeptide chain elongation factor-1 complex with the 26S proteasome during Xenopus oocyte maturation
© Tokumoto et al; licensee BioMed Central Ltd. 2003
Received: 27 May 2003
Accepted: 16 July 2003
Published: 16 July 2003
During Xenopus oocyte maturation, the amount of a 48 kDa protein detected in the 26S proteasome fraction (p48) decreased markedly during oocyte maturation to the low levels seen in unfertilized eggs. The results indicate that the interaction of at least one protein with the 26S proteasome changes during oocyte maturation and early development. An alteration in proteasome function may be important for the regulation of developmental events, such as the rapid cell cycle, in the early embryo. In this study, we identified p48.
p48 was purified by conventional column chromatography. The resulting purified fraction contained two other proteins with molecular masses of 30 (p30) and 37 (p37) kDa. cDNAs encode elongation factor-1γ and δ were obtained by an immuno-screening method using polyclonal antibodies against purified p48 complex, which recognized p48 and p37. N-terminal amino acid sequence analysis of p30 revealed that it was identical to EF-1β. To identify the p48 complex bound to the 26S proteasome as EF-1βγδ, antibodies were raised against the components of purified p48 complex. Recombinant EF-1 β,γ and δ were expressed in Escherichia coli, and an antibody was raised against purified recombinant EF-1γ. Cross-reactivity of the antibodies toward the p48 complex and recombinant proteins showed it to be specific for each component. These results indicate that the p48 complex bound to the 26S proteasome is the EF-1 complex. MPF phosphorylated EF-1γ was shown to bind to the 26S proteasome. When EF-1γ is phosphorylated by MPF, the association is stabilized.
p48 bound to the 26S proteasome is identified as the EF-1γ. EF-1 complex is associated with the 26S proteasome in Xenopus oocytes and the interaction is stabilized by MPF-mediated phosphorylation.
In vertebrates, fully-grown immature oocytes are arrested in late G2 of meiosis I. Secretion of maturation-inducing hormone (MIH) induces the maturation of oocytes and progression of the cell cycle . The mature oocytes arrest at metaphase of meiosis II. Recent evidence indicates that proteolysis plays an important role in regulation of the meiotic and mitotic cell cycles. Among the various components of the cells proteolytic machinery, the ubiquitin-dependent proteolytic system has attracted a great deal of attention . The 26S proteasome is a protease complex of this system . It has been suggested that proteasomes are involved in the regulation of meiotic cell-cycle progression during oocyte maturation . Inhibitor studies suggest that proteasomes may be involved in the early steps of meiotic maturation in animal oocytes corresponding to the G2-M transition [5, 6]. Other evidence for the involvement of proteasomes in meiotic maturation comes from observations that showed modification of subunits in the 26S proteasome during oocyte maturation in fish and frogs [7–9]. The 26S proteasome was also implicated in regulation of exit from meiotic metaphase [10–13]. Together, these results suggest that proteasomes play a crucial role in the meiotic cell cycle of maturing oocytes. However, proteins that are targeted for proteasome-dependent degradation during oocyte maturation have not been investigated in detail.
In a previous study, we examined changes in components of proteasomes during oocyte maturation and early development of Xenopus laevis .Xenopus oocytes are induced to undergo maturation by MIH, which causes G2/M transition. Although no significant changes in the proteins common to 20S and 26S proteasomes were observed during oocyte maturation, the amount of a unique 48 kDa protein detected in the 26S proteasome fraction (p48) decreased markedly during oocyte maturation to the low levels seen in unfertilized eggs. These results indicate that the interaction of at least one protein with the 26S proteasome changes during oocyte maturation and early development. An alteration in proteasome function may be important for the regulation of developmental events, such as the rapid cell cycle, in the early embryo.
We demonstrated the interaction between p48 and the 26S proteasome by several criteria. The p48 polypeptide co-purified with protease activity and with components of the 26S proteasome even using a protocol containing a high-salt treatment. When purified 26S proteasomes were analysed by non-denaturing electrophoresis, p48 was clearly detected in the band corresponding to the 26S proteasome. Furthermore, p48 was immunoprecipitated with the 26S proteasome using a monoclonal antibody raised against the α2 subunit of the 20S proteasome. The results strongly suggested that p48 is associated with the 26S proteasome .
In this study, we identify p48 as a component of eukaryotic polypeptide chain elongation factor-1 βγδ (EF-1βγδ complex), EF-1γ, and demonstrate that the EF-1 complex is bound to the 26S proteasome in Xenopus oocytes. EF-1βγδ complex is involved in polypeptide chain elongation via the GDP/GTP exchange activity of EF-1α . Among the components of EF-1βγδ, EF-1γ has been reported to be a major substrate for maturation-promoting factor (MPF) during oocyte maturation in Xenopus laevis [15–17]. EF-1γ is significantly phosphorylated by MPF during the first and second meiotic metaphase, but its physiological role has not been investigated. In this paper we show that phosphorylation of EF-1γ by MPF stabilizes the interaction with the 26S proteasome.
Identification of p48 complex as EF-1βγδ
Phosphorylation corresponded to disappearance of p48
Phosphorylation of EF-1γ bound to the 26S proteasome by MPF
Phosphorylation of EF-1γ by MPF stabilizes the interaction between EF-1 complex and the 26S proteasome
Changes in interaction between EF-1 complex and the 26S proteasome in vivo
Recently, interactions between the 26S proteasome and other factors have been widely investigated . Subunit 4 (S4) ATPase of the 26S proteasome was identified as a novel E7-binding protein . E7, the human papilloma virus oncoprotein, binds to S4 through the carboxy-terminal zinc-binding motif. The XPB subunit of repair TFIIH is also known to directly interact with SUG1, the 45 kDa subunit of the 26S proteasome . HEC, a protein highly expressed in cancer cells, binds to the 26S proteasome in a cell-cycle dependent manner . The reversible interaction between CCTε and the 26S proteasome has been investigated . A protein kinase, SnRK, has been shown to interact with α4 subunit of the 26S proteasome . Furthermore, various proteasome-interacting proteins were identified by mass spectrometric analysis . These results suggested that particular functions of the 26S proteasome are regulated by specific interactions between factors other than subunits of the 26S proteasome. Likewise, the interaction between EF-1 complex and the 26S proteasome investigated in this study is noteworthy.
Several model structures of EF-1 complex have been put forward [20, 28]. In Xenopus, Minella et al. suggested the model of a protein complex comprised of EF-1β,γ,δ and transfer RNA synthetase . However, they also demonstrated that EF-1 complex was obtained in the high molecular weight fraction (more than 750 kDa) when the cytosolic fraction was directly separated by gel filtration chromatography . Their results suggested that EF-1 complex in Xenopus oocyte is part of the high molecular weight complex. The results of the present study show that EF-1 complex is bound to the 26S proteasome in Xenopus oocytes. This is supported by the fact that isolated EF-1 complex displays some abnormal characteristics. Firstly, EF-1 complex in oocyte extract or in the 26S proteasome fraction can be separated by gel filtration chromatography, but isolated EF-1 complex could not, even using high salt conditions (0.5 M NaCl). This result may be caused by undesirable interaction between the isolated EF-1 complex and the resin (data not shown). Secondly, the isolated EF-1 complex was not a good substrate for MPF, whereas that bound to the 26S proteasome was readily phosphorylated (Fig. 5). These results suggest that EF-1 complex is bound to the 26S proteasome under physiological conditions.
In this study, we demonstrate that EF-1 complex is bound to the 26S proteasome in Xenopus oocytes. Furthermore, we show that phosphorylation by MPF stabilizes the association. Although, the physiological role of the interaction remains to be determined, we favor the notion that the 26S proteasome acts as a positive regulator of the EF-1 complex based on the phosphorylation state of EF-1γ after fertilization. As we showed in a previous study, the intensity of the p48 band remains low until midblastula transition (MBT) . Phosphatase treatment indicates that EF-1γ is highly phosphorylated until MBT. A portion of EF-1 complex may be involved in the synthesis of proteins concerned with rapid cell division. To realize the rapid cell cycle, the EF-1βγδ should be in a complex with the 26S proteasome. Indeed, physiological interactions between proteasomes and ribosomes or ribosome-associated proteins suggest that proteasomes are involved in the regulation of translational processes [27, 30, 31]. Another possibility is that the EF-1 complex may act as a positive regulator of the ubiquitin-dependent proteolytic pathway. EF-1α has been reported to be a positive regulator in the degradation of N-acetylated proteins by the ubiquitin-dependent proteolytic pathway [32, 33]. Recently, the interaction between the 26S proteasome and EF-1α was demonstrated by mass spectrometry analysis of affinity-purified proteasomes . EF-1 complex may stimulate the function of EF-1α. Studies into the physiological roles of the association between the 26S proteasome and EF-1 complex may assist in helping to understand the regulation of protein synthesis and breakdown.
In this study, we demonstrated that EF-1 complex is bound to the 26S proteasome in Xenopus oocytes. Furthermore, we showed that phosphorylation by MPF stabilizes the association. Our results raise the possibility that the 26S proteasome may contribute to the regulation of protein synthesis and breakdown through an interaction with the EF-1 complex.
Purification of p48 complex
The p48 fraction was purified from Xenopus laevis oocyte cytosol prepared from immature oocytes as described below. The 35–80 % ammonium sulfate fractions were prepared from the cytosol. The ion concentration in the dialyzed fraction was adjusted with NaCl equilibrated to 0.3 M NaCl in electric conductivity. The fraction was applied to a Q-Sepharose column (2.6x10 cm) equilibrated with 20 mM Tris-HCl, pH 8.0 (TN buffer) containing 0.3 M NaCl. The column was washed with the same buffer, and proteins were eluted with a linear gradient of NaCl from 0.3 to 0.5 M at a flow rate of 90 ml/hr. Fractions of 10 ml were collected and the amount of p48 was assessed by immunoblotting using anti-20S proteasome polyclonal antibodies. The fractions with the p48 band were pooled and diluted ten fold with TN buffer. Then, the fraction was applied to an SP-Sepharose column (1.6 × 10.0 cm) equilibrated with TN buffer. Adsorbed materials were eluted with a linear gradient of NaCl from 0 to 0.25 M and fractions of 10 ml were collected at a flow rate of 90 ml/hr. Fractions with the p48 band were pooled and concentrated by loading onto a Q-Sepharose column (1.0 × 4.1 cm). The concentrated fractions were pooled as the final purified preparation, frozen in small aliquots and stored at -80°C. All purification procedures were performed at 4°C.
SDS-PAGE and immunoblotting
Proteins were separated by polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE with 12% gel) by the method of Laemmli , and transferred onto Immobilon membranes (Millipore) using a standard protocol. Membranes were blocked in 5% non-fat powdered milk, and incubated with primary antibodies for 1 hr at room temperature. Immunocomplexes were visualized using an ECL detection kit (Amersham Pharmacia Biotech), as described previously .
cDNA cloning by immunoscreening and production of recombinant proteins
Xenopus ovary cDNA library was constructed in the Uni-ZAP XR vector (Stratagene). Using polyclonal antibodies against the purified p48 fraction prepared in this study, immunoscreening was carried out as described previously . From the isolated plaques, plasmid DNA was prepared by the in vivo excision protocol using the ExAssist/SOLR system (Stratagene). DNA sequencing was performed using a 377A DNA sequencer (Perkin Elmer ABI) with a Dye Terminator Cycle Sequencing Kit (Perkin Elmer ABI).
The full-length ORFs of Xenopus EF-1β,γ and δ were cloned into the pET21b expression vector (Novagen) at the Nde I and Xho I sites. These were constructed from each cDNA by the PCR method. The recombinant proteins were produced in E. coli BL21(DE3)pLysE and purified by affinity chromatography on Ni-NTA Agarose (Qiagen) column according to the manufacturer's instructions .
Purification of the 26S proteasome and Peptidase assay
The 26S proteasome was purified from immature Xenopus oocytes as described . Peptidase activity with or without 0.05% SDS was assessed using the fluorogenic peptide substrate, Suc-LLVY-MCA, as described previously .
Antibody production and Immunoprecipitation
Antibodies against purified p48 complex were prepared as described previously . Polyclonal antiserum cross-reactive with p48 and p37 and a monoclonal antibody cross-reactive with p30 (EF-1β) were obtained. A polyclonal antibody against recombinant EF-1γ was prepared as follows. Affinity-purified recombinant EF-1γ (20 μg of protein) in complete Freund's adjuvant was injected into guinea pigs at ten-day intervals until sufficient titer was obtained. The antiserum to Xenopus 20S proteasome and a monoclonal antibody to goldfish 20S proteasome α2 subunit were prepared and used as previously described [7, 38].
Immunoprecipitation was performed with IgG fractions that had been purified from serum on a protein A-Sepharose CL-4B column (Ammersham Pharmacia) as described previously .
Preparation of oocyte and egg extracts
Extracts from Stage VI oocytes (immature oocytes) and ovulated eggs (mature oocytes) were prepared as described previously . Stage VI oocytes were manually isolated from Xenopus ovarian fragments. Ovulated eggs were dejellied with 2% cysteine solution. Groups of twenty oocytes or eggs were washed in MPF extraction buffer (80 mM β-glycerophosphate, 50 mM NaF, 20 mM EGTA, 15 mM MgCl2, 20 mM HEPES, pH 7.5) and transferred to 1.5 ml Eppendorf microcentrifuge tubes. The excess buffer was removed, and 100 μl of fresh buffer was added. The samples were crushed with 5 strokes of a plastic pestle and centrifuged for 10 min at 13,500 rpm at 4°C in a fixed angle rotor (TOMY Model MX-160 microcentrifuge). The clear supernatant (100 μl) was collected for electrophoresis and immunoblotting.
Egg extracts were treated with calf intestine alkaline phosphatase (Boehringer Mannheim, 60 U) for 60 min at 30°C in the presence of protease inhibitors (chymostatin, leupeptin and pepstatin, 500 μg/ml each). Phosphatase-treated egg extracts were assessed by immunoblotting using anti-20S proteasome polyclonal antibodies.
Phosphorylation by MPF
Aliquots (4 μl) of purified p48 complex or the 26S proteasome were incubated in a total volume of 20 μl. The reaction mixture contained 10 mM Tris-HCl, pH 7.5, 1 mM MgCl2, 4 mM 2-mercaptoethanol, 40 μM ATP and 10 μM [γ-32P]-ATP (0.37 Tbq/mmol) with or without 2 units of recombinant human MPF (BIOMOL). Reactions were performed at 30°C for 1 hour and terminated with SDS-PAGE sample buffer. 32P-labeled proteins were resolved by SDS-PAGE and visualized by autoradiography on Imaging plates (Fuji Film).
We are grateful to the staff of the Radiochemistry Research Laboratory and Institute for Genetic Research and Biotechnology of Shizuoka University for the use of equipment. We also thank Y. Makino of the Center for Analytical Instruments of the National Institute for Basic Biology for providing technical assistance in amino acid sequence analysis. This work was supported by Grants-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan and the CREST Research Project of the Japan Science and Technology Corporation to YN. Part of this study was performed as the National Institute for Basic Biology Cooperative Research Program (00-121 to TT).
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