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Table 5 Hydrodynamic analysis of T701-Myo1c in the presence of 5 μM calmodulin

From: Calmodulin binding to recombinant myosin-1c and myosin-1c IQ peptides

Property

Temperature

T701-Myo1c

100 μM EGTA

T701-Myo1c

25 μM CaCl2

Sedimentation coefficient

25°C

4.68 ± 0.11 S (n = 2)

3.95 ± 0.33 S (n = 4)

Molecular mass

25°C

92 ± 4 kD

62 ± 12 kD

Calmodulins per molecule

25°C

2.8 ± 0.2

1.0 ± 0.7

  1. Purified T701-Myo1c co-expressed with calmodulin, was subjected to sucrose-gradient centrifugation in the presence of 5 μM calmodulin in either 100 μM EGTA or 25 μM CaCl2. T701-Myo1c was detected by ELISA. We estimated molecular mass and hence stoichiometry by assuming that Stokes radii of the complexes were unchanged by the presence of calmodulin, an assumption that might have led to an underestimate of the mass. Values reported are mean ± range or standard deviation.
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BMC Biochemistry

ISSN: 1471-2091

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