Table 3 Hydrodynamic analysis of full-length Myo1c

Sedimentation coefficient

4°C

25°C

6.70 ± 0.07 S (n = 5)

6.90 ± 0.08 S (n = 4)

6.28 ± 0.21 S (n = 8)

*

Stokes radius

4°C

25°C

6.06 ± 0.08 nm (n = 7)

6.07 ± 0.05 nm (n = 4)

5.78 ± 0.11 nm (n = 5)

*

Molecular mass

4°C

25°C

168 ± 4 kD

4°C: 173 ± 3 kD

150 ± 7 kD

ND

Frictional ratio

4°C

25°C

1.7 ± 0.1

1.7 ± 0.1

1.7 ± 0.1

ND

Calmodulins per molecule

4°C

25°C

2.5 ± 0.2

2.9 ± 0.2

1.5 ± 0.4

ND