Figure 5From: Osmotic stress-dependent serine phosphorylation of the histidine kinase homologue DokAIn vivo phosphorylation of a PHKR fragment with a disabled catalytic domain. Autoradiography of the PVDF membrane shows that a double mutation in the ATP binding site of PHKR (G1205A, G1207A) does not affect the in vivo phosphorylation of PHKR during osmotic shock (t = 10 min, 30 min).Back to article page