Figure 3From: Osmotic stress-dependent serine phosphorylation of the histidine kinase homologue DokAPhospho-amino acid analysis. Radioactively labeled PHKR was purified by immunoprecipitation from osmotically shocked Dictyostelium cells. The protein was totally hydrolysed by boiling with 6 N HCl at 120°C. Two-dimensional thin-layer electrophoresis was carried out as described [17]. Phospho-serine was detected by autoradiography.Back to article page