Acetylcholinesterase assay for cerebrospinal fluid using bupivacaine to inhibit butyrylcholinesterase

BMC Biochemistry

Table 2 Mean AChE activity ± SEM (nmol/min × ml) in lumbar CSF samples measured with the bupivacaine-inhibition test and the inhibitor-free test.

bupivacaine concentration (mmol/l)	n	AChE activity
		bupivacaine-inhibition test		inhibitor-free test		calculated data: inhibitor-free test result +3%
		nmol/min × ml ± SEM (range)	% measured	nmol/min × ml ± SEM (range)	% defined	nmol/min × ml ± SEM	%
0.1	45	21.6 ± 0.1 (8.7–34.3)	102.8 ± 0.6	20.9 ± 0.0 (9.0–32.8)	100	21.6 ± 1.0 (9.3–33.8)	103
0.2	45	21.5 ± 0.1 (8.6–34.7)	102.4 ± 0.6	20.9 ± 0.0 (9.0–32.8)	100	21.6 ± 1.0 (9.3–33.8)	103
0.5	8	16.6 ± 1.0 (12.6–19.6)	103.4 ± 1.2	16.1 ± 1.0 (12.5–19.6)	100	16.6 ± 1.1 (12.7–20.2)	103

The mean AChE activity measured with the inhibitor-free test was defined as 100% activity. 3% of each individual test result obtained with the inhibitor-free method were added to the original data and the calculated mean AChE activity ± SEM (103%) was displayed.

ISSN: 1471-2091