Figure 4From: Reconstitution of licensed replication origins on Xenopus sperm nuclei using purified proteinsReconstitution of licensing using purified proteins. a, c, d, Licensing activity of different combinations of purified proteins incubated in the presence of 2.5 mM ATP. DNA synthesis is expressed as % of the DNA template replicated. b, Western blot of Mcm7 loaded onto sperm chromatin in whole Xenopus extract, the full reconstituted reaction and the reconstituted reaction lacking RLF-B/Cdt1. The reconstituted reactions contained 2.5 mM ATP.Back to article page