Figure 1From: Reconstitution of licensed replication origins on Xenopus sperm nuclei using purified proteinsFractionation scheme for proteins used in this study. Interphase Xenopus egg extract was fractionated by the methods outlined. See Materials and Methods for more details. Cdc6 was not purified to homogeneity but was replaced in the reconstituted reaction with recombinant material produced in insect cells.Back to article page