Figure 5

Non-denaturing polyacrylamide gel electrophoresis of proteins from induced E. coli. Lanes 1 and 2: non-denaturing polyacrylamide gel electrophoresis of proteins from the supernatant of the cell lysate. Arrows point towards bands that are present in induced E. coli transformed with the Thermoplasma A-ATPase, but absent in the negative control. The sample buffer did not contain DTT or SDS. Lanes 3, 4, 5 and 6 are separations in denaturing SDS polyacrylamide gels. Lane 3: electro-eluted protein from lane 2 (arrow A); lane 4: electro-eluted protein from lane 2 (arrow B); lanes 1 and 5: E. coli Bl21-CodonPlus(DE3)-RIL strain transformed with empty pET-11a vector (negative control); lanes 2 and 6: E. coli Bl21-CodonPlus(DE3)-RIL strain transformed with the Thermoplasma A-ATPase cloned into pET-11a and induced for 8 hours at 37°C.