Figure 4
From: The intein of the Thermoplasma A-ATPase A subunit: Structure, evolution and expression in E. coli
SDS polyacrylamide gel electrophoresis of proteins from induced E. coli. Panel A, Lanes 1 and 3: E. coli Bl21-CodonPlus(DE3)-RIL strain transformed with empty pET-11a vector (negative control); lanes 2, 4, 5 and 6: E. coli Bl21-CodonPlus(DE3)-RIL strain transformed with the Thermoplasma A-ATPase cloned into pET-11a; lane 1-4: cells were induced for 4 hours; lane 5: cells were induced at 16°C for 16 hours; lane 6: cells were induced at 42°C for 2 hours; Sup.: supernatant. Panel B depicts the splicing process schematically. The unprocessed T. acidophilum A-ATPase A subunit has a calculated size of 85 kDalton. The expected molecular weight of the intein is 20 kDalton, and the religated host protein weighs approximately 65 kDalton.