Figure 5
From: Expression, purification and biochemical characterization of Schizosaccharomyces pombe Mcm4, 6 and 7
Gel filtration chromatography profiles of core and near-full-length fragments of Mcm4, 6 and 7. Schematic of each fragment was shown in accordance with its gel filtration profile. Gel filtration analysis was carried out a described under “Methods”. (a) Gel filtration profile of 7 N1 was chosen as a reference, and its dimer peak was used to align with monomer peaks of 7 F4. The other molecular weight shown was determined by Bio-Rad Gel Filtration Standard (data not shown). (b-c) Concentration dependent oligomerization of a core fragment of Mcm4, 4C1. (d) Large and heterogeneous aggregates composed of a nFL fragment of Mcm6, 6 F9. (e-f) Two oligomeric states of a nFL fragment of Mcm7, 7 F4. Peaks on the gel filtration profile correspond to the monomer and the double-hexamer.