Figure 3
From: Expression, purification and biochemical characterization of Schizosaccharomyces pombe Mcm4, 6 and 7
Summary of biochemical properties of fragments of Mcm4, 6 and 7. Schematic of truncated fragments of Mcm4, 6 and 7 tested in this study. The motifs are represented by: “A”-Walker A motif, “B”-Walker B motif, “R”-Arg-finger motif, “Z”-zinc finger motif. The nomenclature for the fragments is as follows, the first numbers represent the Mcm 4, 6, or 7; the letters in the middle indicate domain locations (“N”-N terminal fragments, “C”-core fragments, “F”-near-full-length fragments); the last numbers denotes construct number. a, decreased expression level or plasmid instability; b, oligomeric states depended on protein concentration; c, little equilibrium between monomeric and dimeric states and proteins in the two states could be separated by ion-exchange chromatography; d, a stable large complex identified with a molecular weight equal to a double-hexamer; n/a, not available, due to lack of enough samples.