Figure 2

Designs of truncated fragments of Mcm4, 6 and 7. (A) Schematic of fission yeast Mcm4, 6 and 7. Locations of putative zinc finger (white boxes labeled with Z), the MCM core region (gray boxes) was shown. Three ATPase consensus motifs in the MCM core region were labeled with A (the Walker A motif), B (the Walker B motif) and R (the Arg-finger motif). All conversed amino acid residues that define each motif were shown. All truncation fragments reported in this paper were designed according to three domains, N-terminal, core and C-terminal domains. This figure was generated from the sequence alignment results shown in Additional File 1: Figure S1 and each Mcm protein was aligned with the MCM box region. (B) Disordered profile plot and predicted secondary structure of Mcm4. Only sampled secondary structure prediction was shown and aligned with the disordered profile. A disordered N-termini was present and aligned well with a region (1–150 aa) that lacks any defined secondary structure, while regions with very low disorder probability were predicted to show ordered secondary structures. The disordered profiles were generated by the DISOPRED server, and secondary structure prediction was generated by the PSIPRED server at University College London [15–17]. “Conf”-prediction confidence, “Pred”-predicted secondary structures, “AA”-amino acid residues. Disordered profile plots of Mcm6 and 7 were shown in Additional file 2: Figure S2.