From: A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding
Symmetrical cleavage
Asymmetrical cleavage
Enzyme
6 → 3 + 3
4 → 2 + 2
Total
6 →4 + 2
WT
5,14 (60%)
0,28 (3%)
5,42 (63%)
3,19 (37%)
R42E
5,66 (66%)
0,65 (8%)
6,21 (74%)
2,23 (26%)
R42K
5,73 (65%)
0,53 (6%)
6,26 (71%)
2,60 (29%)