From: A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding
Enzyme
Km(μg/ml)
kcat(min-1)
Ki(μg/ml)
kcat/ Km(min*ml/μg)
Wild-type
26.1
642.1
1547
24.6
R42E
441.7
96.7
263
0.22
R42K
204.1
55.5
612
0.27