Autophosphorylation of serine 608 in the p85 regulatory subunit of wild type or cancer-associated mutants of phosphoinositide 3-kinase does not affect its lipid kinase activity
© Layton et al.; licensee BioMed Central Ltd. 2012
Received: 28 October 2012
Accepted: 22 December 2012
Published: 27 December 2012
The α-isoform of the Type 1A Phosphoinositide 3-kinases (PI3Kα) has protein kinase activity as well as phosphoinositide lipid kinase activity. The best described substrate for its protein kinase activity is its regulatory subunit, p85α, which becomes phosphorylated on Serine 608. Phosphorylation of Serine 608 has been reported to down-regulate its lipid kinase activity.
We have assessed whether oncogenic mutants of PI3Kα, which have up-regulated lipid kinase activity, have altered levels of Serine 608 phosphorylation compared to wild type PI3Kα, and whether differential phosphorylation of Serine 608 contributes to increased activity of oncogenic forms of PI3Kα with point mutations in the helical or the kinase domains. Despite markedly increased lipid kinase activity, protein kinase activity was not altered in oncogenic compared to wild type forms of PI3Kα. By manipulating levels of phosphorylation of Serine 608 in vitro, we found no evidence that the protein kinase activity of PI3Kα affects its phosphoinositide lipid kinase activity in either wild-type or oncogenic mutants of PI3Kα.
Phosphorylation of p85α S608 is not a significant regulator of wild-type or oncogenic PI3Kα lipid kinase activity.
KeywordsPI3K PIK3CA Phosphoinositide Kinase Mutation Oncogene Phosphorylation
Phosphoinositide 3-kinases (PI3Ks) are a ubiquitous family of lipid kinases that catalyse the phosphorylation of phosphoinositide lipids at the 3’ position on the inositide ring . The Class 1A sub-group exists as heterodimers of a p110 catalytic subunit and a p85 regulatory subunit that can phosphorylate three phosphoinositides (PI, PI-(4)-P and PI-(4,5)-P2) in vitro, but are thought to primarily phosphorylate PI-(4,5)-P2in vivo to form PI-(3,4,5)-P3 (PIP3) . PIP3 resides almost exclusively in the plasma membrane and propagates signals by recruiting and activating a variety of downstream proteins that contain PIP3 binding domains (primarily Pleckstrin Homology (PH) domains) . PI3K/PIP3 signalling regulates a wide range of cellular processes including cell growth, survival, glucose metabolism and migration [1, 3].
The prototypic Class 1A PI3K is a heterodimer of the α isoforms of p110 and p85 (p110α/p85α or PI3Kα). Mutations in PIK3CA, the gene encoding p110α, are oncogenic [4–7] and are frequently reported in breast and colon cancers, where they are found in around 25% of human tumours [8–11]. Tumour-associated PIK3CA mutations are all somatic, mono-allelic single base changes that result in single amino acid substitutions. The majority (>80%) of mutations cluster in exon 9 (which codes for the helical domain) or exon 20 (which codes for the kinase domain), most commonly E542K and E545K in exon 9 and H1047R in exon 20 [4, 12]. Cancer-associated, mutated forms of PI3Kα are associated with increased phosphoinositide kinase activity [4–6, 13], leading to up-regulation of downstream signalling events such as phosphorylation of Akt and S6 [5, 14].
Class 1A PI3Ks also have protein kinase activity. The p110α catalytic subunit can phosphorylate its regulatory subunit, p85α, at Serine 608 (S608). Phosphorylation of this site has been reported to result in feed-back inhi-bition by down-modulating the lipid kinase activity of p110α [15–17], however the role of phosphorylation of S608 in signalling by endogenous PI3Kα and the structural mechanism of down-modulation of lipid kinase activity by S608 phosphorylation are not well described. We have assessed the possibility that the increased lipid kinase activities of oncogenic mutants of PI3Kα could be partly due to alterations in the phosphorylation of S608. Rather than make point mutations in S608, which can potentially subtly alter the structure and thus the activity of p110α/p85α heterodimers, we have manipulated the levels of phosphorylation of S608 of highly-purified, recombinant p110α/p85α in vitro and tested the effect on lipid kinase activity. Neither complete dephosphorylation nor a high percentage occupancy of S608 by a phosphate group significantly altered the lipid kinase activity of wild-type PI3Kα. The levels and kinetics of S608 phosphorylation in two oncogenic mutants, E545K and H1047R, were not significantly different to that of wild-type p110α/p85α and the lipid kinase activities of mutant PI3Kα were similarly unaffected by phosphorylation of S608. This suggests that phosphorylation of S608 is not a significant regulator of PI3Kα lipid kinase activity.
Expression, purification and characterisation of recombinant wild-type and mutant PI3Kα
Kinetic analysis of in vitro lipid kinase activity of p110 αEE WT /p85 α, p110 αEE E545K /p85 α and p110 αEE H1047R /p85 α
PI kinase activity a
V max b
35 ± 13c
118 ± 12
150 ± 13
K m (μM)
23 ± 3
22 ± 6
14 ± 7
PI-(4,5)-P 2 kinase activity
35 ± 8
117 ± 13
146 ± 15
K m (μM)
22 ± 5
19 ± 6
9 ± 3
Effect of dephosphorylation of p85α S608 on PI3Kα lipid kinase activity
S608 in the p85 subunit is a substrate for the protein kinase activity of PI3Kα [17, 20]. Phosphorylation of S608 by p110 is reported to down-regulate PI3Kα lipid kinase activity [17, 20]. This raises the possibility that higher lipid kinase activities of oncogenic mutants of PI3Kα could be partly due to alterations in the levels of phosphorylation of S608.
As expected, inclusion of λ phosphatase in kinase assays resulted in no significant incorporation of 32P-labelled phosphate groups into p85α, demonstrating that levels of p85α phosphorylation were low in the presence of λ phosphatase (Figure 2B, upper panel). Dephosphorylated p110αEEWT/p85α did not have a significantly different PI kinase activity to p110αEEWT/p85α that became phosphorylated on p85α over the course of the assay (Figure 2B, lower panel). Similarly, the E545K and H1047R mutants did not have significantly different PI kinase activities when p85α was either dephosphorylated or phosphorylated, although the PI kinase activities of p110αEEE545K/p85α and p110αEEH1047R/p85α were higher than that of p110αEEWT/p85α as expected (Figure 2B, lower panel). Interestingly, as observed previously , the protein kinase activities of p110αEEE545K/p85α and p110αEEH1047R/p85α were not significantly different to that of p110αEEWT/p85α (Figure 2B, upper panel).
It is possible that the stoichiometry of phosphorylation of S608 remains low after the 40 minute kinase reaction used, and thus lipid kinase activities of dephosphorylated and only partially phosphorylated p110αEE/p85α were compared in Figure 2B. We therefore performed a time-course to determine whether a difference in activity of phosphorylated and dephosphorylated p110αEE/p85α became apparent with increasing levels of phosphorylation of p85α. Levels of phosphorylation of S608 became saturated after approximately 30 minutes (Figure 2C, upper panel). In some assays, the level of phosphorylation of the E545K mutant was apparently higher than wild-type PI3Kα or the H1047R mutant, although this was an inconsistent observation and was not significantly different in most assays. When λ phosphatase was added, lack of incorporation of 32P into p85α demonstrated that λ phosphatase continued to dephosphorylate S608 throughout the assay. (Figure 2C, lower panel, closed symbols). Despite apparently saturating phosphorylation of S608 between 40 and 120 minutes, the difference in the PI kinase activity of phosphorylated and dephosphorylated wild-type, E545K or H1047R PI3Kα did not increase over time (Figure 2C, lower panel), suggesting that phosphorylation of S608 does not regulate the PI kinase activity in either wild-type or mutant PI3Kα.
Effect of phosphorylation of p85α S608 on PI3Kα lipid kinase activity
The PI kinase activities of phosphorylated and mock-phosphorylated wild-type PI3Kα were not significantly different over a 2 hour time-course. Similarly, the PI kinase activities of phosphorylated and mock-phosphorylated E545K and H1047R PI3Kα were not significantly different, although the activities of H1047R, and to a lesser extent E545K, were higher than that of wild-type PI3Kα. Nearly stoichiometric phosphorylation of S608 therefore did not affect the PI kinase activity of wild-type or mutant PI3Kα, further suggesting that phosphorylation of S608 does not regulate PI kinase activity.
As reported previously for bovine PI3Kα , we have successfully expressed and purified recombinant complexes of full-length human p110α and p85α, the prototypic form of Class 1A PI3K using the strategy of selecting for the of p110αEE/p85α complex by placing a 6 amino acid epitope tag at the C-terminus of the p110α, the subunit with limiting expression levels. The addition of C-terminal EE tag to p110α did not appear to affect PI3Kα, as recombinant p110αEE/p85α had high stability and the expected activity and substrate specificity. High purity recombinant PI3Kα allowed us to assess the relative phosphoinositide lipid and protein kinase activities of wild-type and two common, tumour-associated mutants of PI3Kα.
Purified, recombinant oncogenic mutant forms of PI3Kα had higher lipid kinase activities compared to wild-type PI3Kα (Figure 1B) as expected [4–6, 13]. The ATP Km was not significantly different between wild-type and mutant forms of PI3Kα (Table 1) as previously reported . However, there was a significant difference in ATP Vmax when either PI or PI-(4,5)-P2 was used as a substrate, in agreement with a previous study . This suggests that mutant forms of PI3Kα phosphorylate and turn over inositol lipids more rapidly, rather than having inherent higher substrate affinities, thus generating increased levels of PIP3 and potentially partially explaining increased downstream signalling when the PI3K pathway is activated.
PI3Kα also has protein kinase activity and has been reported to phosphorylate a number of protein substrates [22, 23] as well as its own p85α regulatory subunit on S608 [16, 17, 20]. The role of S608 phosphorylation in the regulation of PI3Kα lipid kinase activity remains controversial. Despite previous reports that phosphorylation of S608 down-regulates the lipid kinase activity of the p110α catalytic subunit, we did not find any evidence to support this by in vitro manipulation of the levels of phosphorylation of S608 of purified, recombinant p110αEE/p85α using either a protein phosphatase or saturating pre-phosphorylation. The reason for this discrepancy is not clear. In two studies, semi-purified recombinant PI3Kα was used to show phosphorylation of S608 reduces the lipid kinase activity of p110α [17, 20]. Semi-purified PI3Kα can be unstable, thus it is possible that the apparent decrease in the lipid kinase activity of semi-purified PI3Kα over time was due to p110α degradation rather than S608 phosphorylation. A number of other studies have not observed a decrease in lipid kinase activity of recombinant, purified or immunoprecipitated PI3Kα in time-course assays [24–27].
The time-course of PIP3 formation by endogenous PI3K in cells shows an initial increase, followed by a decrease in PIP3 levels [28, 29], but this is largely due to the action of inositol 3- and 5-phosphatases that directly dephosphorylate PIP3 to form PI-(4,5)-P2 or PI-(3.4)-P2 respectively [30, 31]. The phosphorylation of S608 in cells increases upon stimulation with insulin or PDGF , which mirrors activation of the lipid kinase activity of PI3Kα, thus it is not clear whether phosphorylation of S608 down-regulates endogenous PI3Kα activity. Mutation of S608 to a non-phosphorylatable residue has been shown to decrease lipid kinase activity rather than increase it, as would be expected if mutation to alanine prevented phospho-S608-induced down regulation of PI kinase activity , suggesting that mutation of this residue, which resides in the interSH2 domain of p85α, affects PI3Kα lipid kinase activity by altering the structure of PI3Kα rather than by affecting the phosphorylation status.
Phosphorylation of S608 has also been reported to lead to dissociation of the p85α and p110α subunits . Free p110α has higher activity than p110α in complex with p85α but is very unstable and is quickly degraded , thus it is possible that the decrease in lipid kinase activity when p85α S608 is phosphorylated could be explained by dissociation of p110α and p85α and rapid degradation of free p110α. However, our highly purified recombinant forms of PI3Kα, when phosphorylated to saturation on p85α S608, showed no evidence of subunit dissociation by size-exclusion chromatography and no evidence of p110α degradation by SDS-PAGE (data not shown). The lack of observed dissociation, degradation or decrease in lipid kinase activity concomitant with increasing p85α S608 phosphorylation in our highly purified, recombinant PI3Kα suggests that previous reports of S608 phosphorylation resulting in down-regulation of lipid kinase activity were due to the use semi-purified recombinant PI3Kα which is non-specifically degraded over time.
A number of mechanisms that regulate PI3Kα enzymatic activity have been described, but no single event has been demonstrated to result in full activation of this enzyme. It is likely that full activation of PI3Kα is a multi-step process. Within the p110α/p85α complex, p110α is both inhibited and structurally stabilised by tight binding to the p85α subunit . Activation of the PI3Kα involves protein-protein interactions that relieve the inhibition of the p110α kinase activity that is due to inter-subunit interactions of p110α with the N-terminal SH2 and inter-SH2 domains of p85α . One mechanism of activation of PI3Kα is binding to specific phosphotyrosine-containing motifs (pYXXM) present in receptor tyrosine kinases (RTKs) and cytoplasmic signalling proteins, such as IRS-1, to the p85α N- and C-terminal SH2 domains [34–36], which disrupts the inhibitory contact between the p85α N-terminal SH2 and the p110α catalytic domain [37, 38]. The E545K mutation is thought to increase activity by disrupting the p85α N-terminal SH2/p110α interface, similar to binding of RTKs [37, 38]. Binding of a range of other intracellular proteins, such as activated Ras, SH3 domain-containing proteins and small GTPases, have also been reported to activate PI3Kα [39–41] but it is not clear whether binding of these ligands directly activates enzyme activity or whether binding results in activation by translocating PI3Kα to the plasma membrane, where its lipid substrate is located [42, 43]. Tyrosine phosphorylation of p85α Y688 by Src family tyrosine kinases has also been shown to increase PI3Kα activity , but the mechanism is not known.
Phosphorylation of p85α S652 by PKC  (as well as p85α S608 by p110α) has been reported to decrease PI3Kα activity. S608 is in the inter-SH2 domain of p85α, but is not within the section that has been observed to contact p110α [37, 38, 46], therefore it is not clear whether phosphorylation of S608 could influence the inter-subunit interactions of p110α and p85α. A model of the structure of p110 with the N- terminal SH2, C- terminal SH2 and inter-SH2 domains of p85  suggests that the C-terminal part of the inter-SH2 domain (containing S608) and the C-terminal SH2 domain has the potential to contact the catalytic domain of p110. Activation of PI3Kα due to binding of the p85α C-terminal SH2 domain to phosphotyrosine-containing proteins would be expected to be due to a conformational change that disrupts the interface between the p85α C-terminal SH2 domain and the p110α catalytic domain, analogous to the disruption of the inhibitory contact between the p85α N-terminal SH2 and the p110α catalytic domain. In contrast, for phosphorylation of S608 to decrease PI3Kα enzymatic activity, it would have to stabilise this interface and oppose the phosphotyrosine binding-induced disruption. Regulation of PI3Kα by stabilisation of inter-subunit interactions has not yet been demonstrated.
Studies showing a decrease in PI kinase activity of wild-type PI3Kα that correlated with increasing p85α S608 phosphorylation suggested the possibility that decreased S608 phosphorylation of oncogenic PI3Kα could be a mechanism underlying its increased PI kinase activity. This study, in which highly purified, recombinant wild-type and oncogenic, mutant PI3Kα was dephosphorylated or fully phosphorylated on serine 608 in vitro (thus avoiding point mutations which can potentially alter protein structure and activity), found no evidence that phosphorylation of S608 influenced PI kinase activity. In addition, levels of phosphorylation of oncogenic PI3Kα were not different to that of wild-type PI3Kα.
Production of recombinant proteins
cDNAs encoding full-length human p85α (PIK3R1_HUMAN, [Uniprot: P27986], aa1-724) or full-length human wild-type or mutant (E545K or H1047R) p110α (PIK3CA_HUMAN, [Uniprot: P42336], aa1-1068) with an additional C-terminal spacer (PGG) and a Glu- or EE-epitope tag (EFMPME) [18, 48] were subcloned into the transfer plasmid pBlueBac4. Recombinant baculoviruses were produced by co-transfection of these transfer plasmids into Sf9 insect cells with Bac-N-Blue baculovirus DNA (Invitrogen), then plaque purified and amplified as described . EE-epitope tagged p110α (p110αEE) and p85α were co-expressed in exponentially growing Sf9 cells (density = 1.5-2 × 106 cells/ml) by infection with recombinant baculoviruses at a multiplicity of infection (MOI) of between 1 and 10. Wild-type or mutant p110αEE/p85α complexes were purified to homogeneity by anti-EE tag affinity chromatography and anion exchange chromatography as previously described [18, 50]. The concentrations of purified, recombinant PI3Ks were quantified by UV spectroscopy using a molar extinction coefficient of 264115 M-1 cm-1.
Recombinant p110αEE/p85α complexes were separated by SDS-PAGE using 10% Tris-glycine gels. Fast Coomassie Blue staining and destaining was carried out using a microwave oven as described .
Phosphoinositide 3-kinase assays
PI3K assays were carried out essentially as previously described [18, 52, 53] in 20 mM Tris pH7.5, 150 mM NaCl (TBS) containing 5 mM 2-mercaptoethanol. PI kinase assays contained 2 mM MgCl2, 2 mM MnCl2, 0.2 mM ATP, 5–10 μCi 32P]γATP, 500 μg/ml of phosphatidylinositol (PI) and 250 μg/ml of phosphatidylserine (PS). Extracted phospholipids were separated by thin layer chromatography in 65% 1-propanol, 0.7 M acetic acid, 50 mM phosphoric acid, exposed to a phosphor screen (Molecular Dynamics) and analysed using ImageQuant software (GE Healthcare). Michaelis-Menten kinetics for phosphorylation of PI and PI-(4,5)-P2 were calculated from initial reaction rates in assays in which the concentration of ATP was varied between 0 and 125 μM. Reactions were stopped after 20 min using 1 M HCl.
Protein kinase assays
Protein kinase assays were carried out in TBS containing 2 mM MgCl2, 2 mM MnCl2, 0.2 mM ATP and 5–10 μCi [32P]γATP. Phosphorylated proteins were separated from free [32P]γATP by SDS-PAGE as described above. Gels were fixed and stained with Coomassie Blue, then dried, exposed to a phosphor screen and analysed as described above.
500 ng recombinant PI3K was mixed with 2–10 U recombinant Calf Intestinal Alkaline Phosphatase, 0.5-2 U Antarctic Phosphatase or 8–40 U Lambda (λ) Protein Phosphatase (NEB) and assayed for PI kinase activity or protein kinase activity as described above.
In vitro phosphorylation of recombinant p110αEE/p85α
25 μg purified wild type or mutant p110αEE/p85α complex was incubated with or without 1 mM ATP in the presence of 20 mM Tris pH 7.5, 150 mM NaCl, 5 mM 2-mercaptoethanol, 2 mM MgCl2 and 2 mM MnCl2 at room temperature for 16 hr. Phosphorylated or mock phosphorylated PI3Kα was buffer exchanged into TBS containing 10 mM 2-mercaptoethanol using Sephadex G-25 (GE Life Sciences) and concentrated using centrifugal filters (Amicon Ultra15 10,000 NMWL, Millipore). 250 ng of phosphorylated or mock phosphorylated (control) PI3Kα was assayed for PI kinase activity or protein kinase activity as described above.
Phosphorylation sites on p110αEE/p85α were mapped by digesting 2 μg aliquots of phosphorylated or mock phosphorylated p110αEE/p85α with 0.1 μg trypsin (Worthington) then purifying phosphopeptides using Fe3+ immobilised metal affinity chromatography (IMAC) as described . Phosphopeptides were identified by peptide mass fingerprinting using a QSTAR oMALDI-QqTOF (Applied Biosystems/PE Sciex).
This research was supported by a Grant-in-Aid from the Cancer Council Victoria.
- Vanhaesebroeck B, Stephens L, Hawkins P: PI3K signalling: the path to discovery and understanding. Nat Rev Mol Cell Biol. 2012, 13 (3): 195-203. 10.1038/nrm3290.PubMedView ArticleGoogle Scholar
- Lemmon MA: Membrane recognition by phospholipid-binding domains. Nat Rev Mol Cell Biol. 2008, 9 (2): 99-111. 10.1038/nrm2328.PubMedView ArticleGoogle Scholar
- Cantley LC: The phosphoinositide 3-kinase pathway. Science. 2002, 296 (5573): 1655-1657. 10.1126/science.296.5573.1655.PubMedView ArticleGoogle Scholar
- Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, Yan H, Gazdar A, Powell SM, Riggins GJ, Willson JK, Markowitz S, Kinzler KW, Vogelstein B, Velculescu VE: High frequency of mutations of the PIK3CA gene in human cancers. Science. 2004, 304 (5670): 554-10.1126/science.1096502.PubMedView ArticleGoogle Scholar
- Kang S, Bader AG, Vogt PK: Phosphatidylinositol 3-kinase mutations identified in human cancer are oncogenic. Proc Natl Acad Sci U S A. 2005, 102 (3): 802-807. 10.1073/pnas.0408864102.PubMedPubMed CentralView ArticleGoogle Scholar
- Isakoff SJ, Engelman JA, Irie HY, Luo J, Brachmann SM, Pearline RV, Cantley LC, Brugge JS: Breast cancer-associated PIK3CA mutations are oncogenic in mammary epithelial cells. Cancer Res. 2005, 65 (23): 10992-11000. 10.1158/0008-5472.CAN-05-2612.PubMedView ArticleGoogle Scholar
- Bader AG, Kang S, Vogt PK: Cancer-specific mutations in PIK3CA are oncogenic in vivo. Proc Natl Acad Sci U S A. 2006, 103 (5): 1475-1479. 10.1073/pnas.0510857103.PubMedPubMed CentralView ArticleGoogle Scholar
- Bachman KE, Argani P, Samuels Y, Silliman N, Ptak J, Szabo S, Konishi H, Karakas B, Blair BG, Lin C, Peters BA, Velculescu VE, Park BH: The PIK3CA gene is mutated with high frequency in human breast cancers. Cancer Biol Ther. 2004, 3 (8): 772-775. 10.4161/cbt.3.8.994.PubMedView ArticleGoogle Scholar
- Campbell IG, Russell SE, Choong DY, Montgomery KG, Ciavarella ML, Hooi CS, Cristiano BE, Pearson RB, Phillips WA: Mutation of the PIK3CA gene in ovarian and breast cancer. Cancer Res. 2004, 64 (21): 7678-7681. 10.1158/0008-5472.CAN-04-2933.PubMedView ArticleGoogle Scholar
- Velho S, Oliveira C, Ferreira A, Ferreira AC, Suriano G, Schwartz S, Duval A, Carneiro F, Machado JC, Hamelin R, Seruca R: The prevalence of PIK3CA mutations in gastric and colon cancer. Eur J Cancer. 2005, 41 (11): 1649-1654. 10.1016/j.ejca.2005.04.022.PubMedView ArticleGoogle Scholar
- Miyaki M, Iijima T, Yamaguchi T, Takahashi K, Matsumoto H, Yasutome M, Funata N, Mori T: Mutations of the PIK3CA gene in hereditary colorectal cancers. Int J Cancer. 2007, 121 (7): 1627-1630. 10.1002/ijc.22829.PubMedView ArticleGoogle Scholar
- Liu P, Cheng H, Roberts TM, Zhao JJ: Targeting the phosphoinositide 3-kinase pathway in cancer. Nat Rev Drug Discov. 2009, 8 (8): 627-644. 10.1038/nrd2926.PubMedPubMed CentralView ArticleGoogle Scholar
- Ikenoue T, Kanai F, Hikiba Y, Obata T, Tanaka Y, Imamura J, Ohta M, Jazag A, Guleng B, Tateishi K, Asaoka Y, Matsumura M, Kawabe T, Omata M: Functional analysis of PIK3CA gene mutations in human colorectal cancer. Cancer Res. 2005, 65 (11): 4562-4567. 10.1158/0008-5472.CAN-04-4114.PubMedView ArticleGoogle Scholar
- Zhao JJ, Liu Z, Wang L, Shin E, Loda MF, Roberts TM: The oncogenic properties of mutant p110alpha and p110beta phosphatidylinositol 3-kinases in human mammary epithelial cells. Proc Natl Acad Sci U S A. 2005, 102 (51): 18443-18448. 10.1073/pnas.0508988102.PubMedPubMed CentralView ArticleGoogle Scholar
- Chaussade C, Cho K, Mawson C, Rewcastle GW, Shepherd PR: Functional differences between two classes of oncogenic mutation in the PIK3CA gene. Biochem Biophys Res Commun. 2009, 381 (4): 577-581. 10.1016/j.bbrc.2009.02.081.PubMedView ArticleGoogle Scholar
- Foukas LC, Beeton CA, Jensen J, Phillips WA, Shepherd PR: Regulation of phosphoinositide 3-kinase by its intrinsic serine kinase activity in vivo. Mol Cell Biol. 2004, 24 (3): 966-975. 10.1128/MCB.24.3.966-975.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Dhand R, Hiles I, Panayotou G, Roche S, Fry MJ, Gout I, Totty NF, Truong O, Vicendo P, Yonezawa K: PI 3-kinase is a dual specificity enzyme: autoregulation by an intrinsic protein-serine kinase activity. EMBO J. 1994, 13 (3): 522-533.PubMedPubMed CentralGoogle Scholar
- Layton MJ, Harpur AG, Panayotou G, Bastiaens PI, Waterfield MD: Binding of a diphosphotyrosine-containing peptide that mimics activated platelet-derived growth factor receptor beta induces oligomerization of phosphatidylinositol 3-kinase. J Biol Chem. 1998, 273 (50): 33379-33385. 10.1074/jbc.273.50.33379.PubMedView ArticleGoogle Scholar
- Carson JD, Van Aller G, Lehr R, Sinnamon RH, Kirkpatrick RB, Auger KR, Dhanak D, Copeland RA, Gontarek RR, Tummino PJ, Luo L: Effects of oncogenic p110alpha subunit mutations on the lipid kinase activity of phosphoinositide 3-kinase. Biochem J. 2008, 409 (2): 519-524. 10.1042/BJ20070681.PubMedView ArticleGoogle Scholar
- Carpenter CL, Auger KR, Duckworth BC, Hou WM, Schaffhausen B, Cantley LC: A tightly associated serine/threonine protein kinase regulates phosphoinositide 3-kinase activity. Mol Cell Biol. 1993, 13 (3): 1657-1665.PubMedPubMed CentralView ArticleGoogle Scholar
- Layton MJ, Church NL, Faux MC, Ji H, Goode RJ, Kapp EA, Burgess AW, Simpson RJ: Solubilisation of the armadillo-repeat protein beta-catenin using a zwitterionic detergent allows resolution of phosphorylated forms by 2DE. Electrophoresis. 2012, 33 (12): 1804-1813. 10.1002/elps.201100671.PubMedView ArticleGoogle Scholar
- Lam K, Carpenter CL, Ruderman NB, Friel JC, Kelly KL: The phosphatidylinositol 3-kinase serine kinase phosphorylates IRS-1. Stimulation by insulin and inhibition by wortmannin. J Biol Chem. 1994, 269 (32): 20648-20652.PubMedGoogle Scholar
- Foukas LC, Shepherd PR: eIF4E Binding protein 1 and H-Ras are novel substrates for the protein kinase activity of class-I phosphoinositide 3-kinase. Biochem Biophys Res Commun. 2004, 319 (2): 541-549. 10.1016/j.bbrc.2004.04.191.PubMedView ArticleGoogle Scholar
- Beeton CA, Chance EM, Foukas LC, Shepherd PR: Comparison of the kinetic properties of the lipid- and protein-kinase activities of the p110alpha and p110beta catalytic subunits of class-Ia phosphoinositide 3-kinases. Biochem J. 2000, 350 (Pt 2): 353-359.PubMedPubMed CentralView ArticleGoogle Scholar
- Ruiz-Larrea F, Vicendo P, Yaish P, End P, Panayotou G, Fry MJ, Morgan SJ, Thompson A, Parker PJ, Waterfield MD: Characterization of the bovine brain cytosolic phosphatidylinositol 3-kinase complex. Biochem J. 1993, 290 (Pt 2): 609-616.PubMedPubMed CentralView ArticleGoogle Scholar
- Michell RH, Harwood JL, Coleman R, Hawthorne JN: Characteristics of rat liver phosphatidylinositol kinase and its presence in the plasma membrane. Biochim Biophys Acta. 1967, 144 (3): 649-658. 10.1016/0005-2760(67)90053-7.PubMedView ArticleGoogle Scholar
- Van Aller GS, Carson JD, Fernandes C, Lehr R, Sinnamon RH, Kirkpatrick RB, Tummino PJ, Luo L: Characterization of PI3K class IA isoforms with regulatory subunit p55alpha using a scintillation proximity assay. Anal Biochem. 2008, 383 (2): 311-315. 10.1016/j.ab.2008.08.037.PubMedView ArticleGoogle Scholar
- Stephens L, Jackson T, Hawkins PT: Synthesis of phosphatidylinositol 3,4,5-trisphosphate in permeabilized neutrophils regulated by receptors and G-proteins. J Biol Chem. 1993, 268 (23): 17162-17172.PubMedGoogle Scholar
- van der Kaay J, Batty IH, Cross DA, Watt PW, Downes CP: A novel, rapid, and highly sensitive mass assay for phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) and its application to measure insulin-stimulated PtdIns(3,4,5)P3 production in rat skeletal muscle in vivo. J Biol Chem. 1997, 272 (9): 5477-5481. 10.1074/jbc.272.9.5477.PubMedView ArticleGoogle Scholar
- Sasaki T, Takasuga S, Sasaki J, Kofuji S, Eguchi S, Yamazaki M, Suzuki A: Mammalian phosphoinositide kinases and phosphatases. Prog Lipid Res. 2009, 48 (6): 307-343. 10.1016/j.plipres.2009.06.001.PubMedView ArticleGoogle Scholar
- Dyson JM, Fedele CG, Davies EM, Becanovic J, Mitchell CA: Phosphoinositide phosphatases: just as important as the kinases. Sub-cellular biochemistry. 2012, 58: 215-279. 10.1007/978-94-007-3012-0_7.PubMedView ArticleGoogle Scholar
- Yu J, Zhang Y, McIlroy J, Rordorf-Nikolic T, Orr GA, Backer JM: Regulation of the p85/p110 phosphatidylinositol 3’-kinase: stabilization and inhibition of the p110alpha catalytic subunit by the p85 regulatory subunit. Mol Cell Biol. 1998, 18 (3): 1379-1387.PubMedPubMed CentralView ArticleGoogle Scholar
- Yu J, Wjasow C, Backer JM: Regulation of the p85/p110alpha phosphatidylinositol 3’-kinase. Distinct roles for the n-terminal and c-terminal SH2 domains. J Biol Chem. 273, 273 (46): 30199-30203.View ArticleGoogle Scholar
- Backer JM, Myers MG, Shoelson SE, Chin DJ, Sun XJ, Miralpeix M, Hu P, Margolis B, Skolnik EY, Schlessinger J: Phosphatidylinositol 3’-kinase is activated by association with IRS-1 during insulin stimulation. EMBO J. 1992, 11 (9): 3469-3479.PubMedPubMed CentralGoogle Scholar
- Rordorf-Nikolic T, Van Horn DJ, Chen D, White MF, Backer JM: Regulation of phosphatidylinositol 3’-kinase by tyrosyl phosphoproteins. Full activation requires occupancy of both SH2 domains in the 85-kDa regulatory subunit. J Biol Chem. 1995, 270 (8): 3662-3666. 10.1074/jbc.270.8.3662.PubMedView ArticleGoogle Scholar
- Carpenter CL, Auger KR, Chanudhuri M, Yoakim M, Schaffhausen B, Shoelson S, Cantley LC: Phosphoinositide 3-kinase is activated by phosphopeptides that bind to the SH2 domains of the 85-kDa subunit. J Biol Chem. 1993, 268 (13): 9478-9483.PubMedGoogle Scholar
- Miled N, Yan Y, Hon WC, Perisic O, Zvelebil M, Inbar Y, Schneidman-Duhovny D, Wolfson HJ, Backer JM, Williams RL: Mechanism of two classes of cancer mutations in the phosphoinositide 3-kinase catalytic subunit. Science. 2007, 317 (5835): 239-242. 10.1126/science.1135394.PubMedView ArticleGoogle Scholar
- Mandelker D, Gabelli SB, Schmidt-Kittler O, Zhu J, Cheong I, Huang CH, Kinzler KW, Vogelstein B, Amzel LM: A frequent kinase domain mutation that changes the interaction between PI3Kalpha and the membrane. Proc Natl Acad Sci U S A. 2009, 106 (40): 16996-17001. 10.1073/pnas.0908444106.PubMedPubMed CentralView ArticleGoogle Scholar
- Rodriguez-Viciana P, Warne PH, Dhand R, Vanhaesebroeck B, Gout I, Fry MJ, Waterfield MD, Downward J: Phosphatidylinositol-3-OH kinase as a direct target of Ras. Nature. 1994, 370 (6490): 527-532. 10.1038/370527a0.PubMedView ArticleGoogle Scholar
- Pleiman CM, Hertz WM, Cambier JC: Activation of phosphatidylinositol-3’ kinase by Src-family kinase SH3 binding to the p85 subunit. Science. 1994, 263 (5153): 1609-1612. 10.1126/science.8128248.PubMedView ArticleGoogle Scholar
- Tolias KF, Cantley LC, Carpenter CL: Rho family GTPases bind to phosphoinositide kinases. J Biol Chem. 1995, 270 (30): 17656-17659. 10.1074/jbc.270.30.17656.PubMedView ArticleGoogle Scholar
- Klippel A, Reinhard C, Kavanaugh WM, Apell G, Escobedo MA, Williams LT: Membrane localization of phosphatidylinositol 3-kinase is sufficient to activate multiple signal-transducing kinase pathways. Mol Cell Biol. 1996, 16 (8): 4117-4127.PubMedPubMed CentralView ArticleGoogle Scholar
- Rodriguez-Viciana P, Warne PH, Vanhaesebroeck B, Waterfield MD, Downward J: Activation of phosphoinositide 3-kinase by interaction with Ras and by point mutation. EMBO J. 1996, 15 (10): 2442-2451.PubMedPubMed CentralGoogle Scholar
- von Willebrand M, Williams S, Saxena M, Gilman J, Tailor P, Jascur T, Amarante-Mendes GP, Green DR, Mustelin T: Modification of phosphatidylinositol 3-kinase SH2 domain binding properties by Abl- or Lck-mediated tyrosine phosphorylation at Tyr-688. J Biol Chem. 1998, 273 (7): 3994-4000. 10.1074/jbc.273.7.3994.PubMedView ArticleGoogle Scholar
- Lee JY, Chiu YH, Asara J, Cantley LC: Inhibition of PI3K binding to activators by serine phosphorylation of PI3K regulatory subunit p85alpha Src homology-2 domains. Proc Natl Acad Sci U S A. 2011, 108 (34): 14157-14162. 10.1073/pnas.1107747108.PubMedPubMed CentralView ArticleGoogle Scholar
- Huang CH, Mandelker D, Schmidt-Kittler O, Samuels Y, Velculescu VE, Kinzler KW, Vogelstein B, Gabelli SB, Amzel LM: The structure of a human p110alpha/p85alpha complex elucidates the effects of oncogenic PI3Kalpha mutations. Science. 2007, 318 (5857): 1744-1748. 10.1126/science.1150799.PubMedView ArticleGoogle Scholar
- Vadas O, Burke JE, Zhang X, Berndt A, Williams RL: Structural basis for activation and inhibition of class I phosphoinositide 3-kinases. Sci Signal. 2011, 4 (195): re2-10.1126/scisignal.2002165.PubMedView ArticleGoogle Scholar
- Porfiri E, Evans T, Chardin P, Hancock JF: Prenylation of Ras proteins is required for efficient hSOS1-promoted guanine nucleotide exchange. J Biol Chem. 1994, 269 (36): 22672-22677.PubMedGoogle Scholar
- O’Reilly DR, Miller LK, Luckow VA: Baculovirus expression vectors: a laboratory manual. 1994, Oxford University Press, OxfordGoogle Scholar
- Catimel B, Layton M, Church N, Ross J, Condron M, Faux M, Simpson RJ, Burgess AW, Nice EC: In situ phosphorylation of immobilized receptors on biosensor surfaces: application to E-cadherin/beta-catenin interactions. Anal Biochem. 2006, 357 (2): 277-288. 10.1016/j.ab.2006.07.034.PubMedView ArticleGoogle Scholar
- Wong C, Sridhara S, Bardwell JC, Jakob U: Heating greatly speeds coomassie blue staining and destaining. Biotechniques. 2000, 28 (3): 426-428. 430, 432PubMedGoogle Scholar
- Whitman M, Kaplan DR, Schaffhausen B, Cantley L, Roberts TM: Association of phosphatidylinositol kinase activity with polyoma middle-T competent for transformation. Nature. 1985, 315 (6016): 239-242. 10.1038/315239a0.PubMedView ArticleGoogle Scholar
- Arcaro A, Volinia S, Zvelebil MJ, Stein R, Watton SJ, Layton MJ, Gout I, Ahmadi K, Downward J, Waterfield MD: Human phosphoinositide 3-kinase C2beta, the role of calcium and the C2 domain in enzyme activity. J Biol Chem. 1998, 273 (49): 33082-33090. 10.1074/jbc.273.49.33082.PubMedView ArticleGoogle Scholar
- Stensballe A, Steen H, Jensen ON: Proteomic methods for phosphorylation site mapping. Proteins and proteomics: a laboratory manual. Edited by: Simpson RJ. 2003, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 926-xiiiGoogle Scholar
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