| pH 7.0 |  | pH 8.0 |  |
---|
Protein
|
P
50
|
n
|
P
50
|
n
|
HbA | 1.7 ± 0.1 | 2.05 ± 0.02 | 0.39 ± 0.3 | 1.72 ± 0.03 |
PEG-Hboxy | 0.4 [35] | 1.2 [36] | Â | Â |
Tb Hb | 28.2 ± 0.2 | 2.06 ± 0.01 | 7.3 ± 0.1 | 1.75 ± 0.03 |
PEGTb Hb | 19.7 ± 0.3 | 1.33 ± 0.02 | 5.3 ± 0.1 | 1.09 ± 0.02 |
PEGTb Hb + ATP | 31.1 ± 0.2 | 1.09 ± 0.01 | 4.4 ± 0.3 | 1.02 ± 0.03 |
- Measurements were made in 100 mM HEPES, 1 mM EDTA, 5 mM ascorbate, 103 U/ml catalase, at 10°C. The values for PEG-Hboxy were calculated from published data taking into account the temperature dependence of the oxygen binding parameters.