Figure 5
CO rebinding properties of derivatized hemoglobins. Effect of PEGylation on CO rebinding to HbA and Tb Hb and their PEGylated derivatives. The time courses of the deoxyheme fraction are shown for HbA (gray circles), PEG-Hboxy (black circles), Tb Hb (gray solid line) and PEGTb Hb (black solid line), in 100 mM HEPES, 1 mM sodium EDTA, 1 atm CO, pH 7.0 at 10°C. Data were fitted as reported in Materials and Methods. The fitting curves are shown in red.