Table 4 Kinetic parameters for hydrolysis of pNPPC.
Enzyme | Km (μM) | Vmax (μM/sec) (× 10-2) | kcat (sec-1) | kcat/Km (M-1sec-1) (× 104) |
|---|---|---|---|---|
L107F | 310 ± 5.0 | 1.4 ± 0.05 | 1.7 ± 0.1 | 0.60 ± 0.02 |
Wild type | 360 ± 8.0 | 1.1 ± 0.01 | 1.4 ± 0.1 | 0.40 ± 0.03 |
Y142A | 400 ± 4.0 | 1.4 ± 0.01 | 1.7 ± 0.1 | 0.40 ± 0.02 |
F80A | 450 ± 10.0 | 0.63 ± 0.04 | 0.8 ± 0.1 | 0.20 ± 0.1 |
E169A | 170 ± 30.0 | 0.42 ± 0.03 | 0.5 ± 0.03 | 0.30 ± 0.02 |
E169Q | 440 ± 5.0 | 1.2 ± 0.02 | 1.4 ± 0.1 | 0.30 ± 0.02 |
Y166A | 820 ± 20.0 | 0.62 ± 0.03 | 0.7 ± 0.04 | 0.09 ± 0.01 |
F275A | 120 ± 5.0 | 0.23 ± 0.03 | 0.3 ± 0.03 | 0.03 ± 0.01 |