Table 3 Kinetic parameters for hydrolysis of SM.
Enzyme | Km (μM) | Vmax (μM/sec) (× 10-2) | kcat (sec-1) | kcat/Km (M-1sec-1) (× 104) |
|---|---|---|---|---|
E169Q | 15 ± 5 | 3.2 ± 0.08 | 3.8 ± 0.1 | 25.0 ± 6.0 |
Y166A | 12 ± 0.4 | 2.6 ± 0.01 | 3.1 ± 0.2 | 25.8 ± 5.0 |
F80A | 14 ± 6 | 2.2 ± 0.3 | 2.6 ± 0.3 | 18.6 ± 5.0 |
Wild type | 23 ± 3 | 3.0 ± 0.1 | 3.7 ± 0.2 | 16.5 ± 4.0 |
E169A | 17 ± 2 | 1.8 ± 0.1 | 2.2 ± 0.2 | 11.6 ± 2.0 |
L107F | 37 ± 3 | 3.5 ± 0.1 | 4.2 ± 0.1 | 11.4 ± 4.0 |
Y142A | 47 ± 7 | 3.1 ± 0.2 | 3.7 ± 0.2 | 7.9 ± 3.0 |
F275A | 12 ± 1 | 0.7 ± 0.1 | 0.9 ± 0.2 | 7.5 ± 2.0 |