Enzyme | Km (μM) | Vmax (μM/sec) (× 10-2) | kcat (sec-1) | kcat/Km (M-1sec-1) (× 104) |
---|---|---|---|---|
Y142A | 75 ± 2 | 2.0 ± 0.03 | 2.5 ± 0.04 | 3.3 ± 0.6 |
L107F | 94 ± 3 | 2.4 ± 0.3 | 2.9 ± 0.3 | 3.1 ± 0.2 |
Wild type | 82 ± 2 | 1.3 ± 0.02 | 1.6 ± 0.02 | 2.0 ± 0.8 |
E169Q | 130 ± 8 | 1.9 ± 0.1 | 2.3 ± 0.2 | 1.7 ± 0.2 |
F80A | 120 ± 3 | 1.2 ± 0.03 | 1.4 ± 0.03 | 1.2 ± 0.4 |
E169A | 95 ± 6 | 0.8 ± 0.02 | 0.80 ± 0.02 | 1.0 ± 0.2 |
Y166A | 200 ± 10 | 0.7 ± 0.02 | 0.80 ± 0.04 | 0.4 ± 0.2 |
F275A | No activity | Â | Â | Â |