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Table 2 Kinetic parameters for hydrolysis of PAF 16:0.

From: Computational identification and experimental characterization of substrate binding determinants of nucleotide pyrophosphatase/phosphodiesterase 7

Enzyme

Km (μM)

Vmax (μM/sec)

(× 10-2)

kcat (sec-1)

kcat/Km (M-1sec-1) (× 104)

Y142A

75 ± 2

2.0 ± 0.03

2.5 ± 0.04

3.3 ± 0.6

L107F

94 ± 3

2.4 ± 0.3

2.9 ± 0.3

3.1 ± 0.2

Wild type

82 ± 2

1.3 ± 0.02

1.6 ± 0.02

2.0 ± 0.8

E169Q

130 ± 8

1.9 ± 0.1

2.3 ± 0.2

1.7 ± 0.2

F80A

120 ± 3

1.2 ± 0.03

1.4 ± 0.03

1.2 ± 0.4

E169A

95 ± 6

0.8 ± 0.02

0.80 ± 0.02

1.0 ± 0.2

Y166A

200 ± 10

0.7 ± 0.02

0.80 ± 0.04

0.4 ± 0.2

F275A

No activity

  Â