Table 1 Kinetic parameters for hydrolysis of LPC 16:0.
Enzyme | Km (μM) | Vmax (μM/sec) (× 10-2) | kcat(sec-1) | kcat/Km (M-1sec-1) (× 104) |
|---|---|---|---|---|
L107F | 61 ± 2.8 | 3.5 ± 0.03 | 4.2 ± 0.03 | 6.8 ± 1.6 |
Y142A | 69 ± 0.9 | 3.0 ± 0.01 | 3.6 ± 0.01 | 5.2 ± 0.05 |
Wild type | 57 ± 0.7 | 2.5 ± 0.01 | 2.9 ± 0.01 | 5.1 ± 0.1 |
E169Q | 88 ± 6.8 | 3.3 ± 0.02 | 3.8 ± 0.03 | 4.3 ± 0.4 |
E169A | 100 ± 15 | 1.0 ± 0.04 | 1.2 ± 0.05 | 1.2 ± 0.2 |
F80A | 120 ± 3.0 | 0.7 ± 0.01 | 1.4 ± 0.01 | 1.2 ± 0.3 |
Y166A | 100 ± 5.0 | 0.9 ± 0.03 | 1.1 ± 0.03 | 1.0 ± 0.2 |
F275A | No activity | Â | Â | Â |