Figure 5
Transcription coupled repair (TCR) was detected by the strand-specific PCR, and CSB depletion led to decreased efficiency of TCR. A & B: Transfection of specific siRNA-expressing vectors (CSBsiRNA-52 and CSBsiRNA-107) mediated depression of the CSB gene in HeLa cells, detected by semi-quantitative RT-PCR assay (A) and immunoblotting assay (B). C: A representative strand specific PCR to assay TCR in CSB depleted HeLasiRNA-107 and the control HeLa-NC cells. Depression of CSB resulted in a decreased repair efficiency of the DHFR gene transcribed-strand DNA damage induced by UV, but there was no influence on the untranscribed strand DNA damage repair. Cells were collected at 0, 2, 4, 8 and 24 h after 10 J/m2 UV radiation, then TCR efficiency was assayed by SS-PCR as described in method.