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Figure 1 | BMC Biochemistry

Figure 1

From: Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex

Figure 1

Single step fluorescent detection of SBP-tagged SMC2. Cyto-centrifuged DT40 wild-type cells (A) and SMC2-SBP cells (B, C) were incubated with streptavidin-488 for single-step fluorescence analysis. Mitotic chromosomes from DT40 wild-type cells show no staining, whilst chromosomes from SMC2-SBP display the distinct characteristic chromosome scaffold staining pattern of condensin subunits. Note also in (C) that the interphase nuclei display weak diffuse staining of SMC2-SBP cells which is also consistent with known condensin antibody staining [8].

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BMC Biochemistry

ISSN: 1471-2091

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