Figure 1From: The DNA relaxation activity and covalent complex accumulation of Mycobacterium tuberculosis topoisomerase I can be assayed in Escherichia coli: application for identification of potential FRET-dye labeling sitesComplementation of topAts function in E. coli AS17 by recombinant M. tuberculosis topoisomerase I. Transformants of E. coli AS17 with pMTOP (M), pETOP (E) and vector pBAD/thio (V) were streaked on LB plates with 100 μg/ml ampicillin and incubated at the indicated temperature for 48 h.Back to article page