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Table 2 Percentage of turbiditya of the heat-denatured substrates

From: Investigation of the chaperone function of the small heat shock protein — AgsA

 

MDH

CS

 

50°C

60°C

42°C

50°C

60°C

AgsA

3.2 ± 1.3

6.0 ± 0.3

0.4 ± 0.7

0.1 ± 0.1

2.7 ± 0.7

ΔN11

5.9 ± 1.5

40.1 ± 3.0

1.2 ± 2.1

0.6 ± 0.2

0.7 ± 0.3

ΔN17

87.3 ± 1.5

103.0 ± 2.4

0.6 ± 0.5

0.3 ± 0.2

1.9 ± 0.5

ΔC11

5.0 ± 2.6

81.5 ± 0.8

2.7 ± 2.5

39.8 ± 5.2

193.7 ± 15.3

  1. aThe percentage of turbidity showed the ratio of the turbidity of heat-denatured MDH (5 μM) or CS (1.5 μM) with 10 μM AgsA or its mutants to the turbidity of heat-denatured MDH or CS alone (for details, see the Materials and Methods section). Values are the mean ± SD obtained from 3 independent experiments.
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BMC Biochemistry

ISSN: 1471-2091

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