Figure 6
From: Investigation of the chaperone function of the small heat shock protein — AgsA
Measurement of the interaction between AgsA and ΔC11. (A) Equal amounts (50 μM) AgsA and ΔC11 were mixed and immediately (Black line) or after 1 h incubation at 25°C (red line) analysed using size exclusion chromatography at 25°C. The retention volume of the protein molecular weight standards is shown above the panel. (B) Fluorescein-labelled AgsA and rhodamine-labelled AgsA (solid circles), fluorescein-labelled ΔC11 and rhodamine-labelled AgsA (open diamond), fluorescein-labelled AgsA and rhodamine-labelled ΔC11 (open triangles), and fluorescein-labelled ΔC11 and rhodamine-labelled ΔC11 (solid squares) were mixed and incubated for the subunit exchange reaction at 25°C. The rate of subunit exchange is reflected by the time-dependent change for the emission fluorescence intensity of fluorescein recorded at 525 nm [F525 nm(t)/F525 nm(0)].