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Figure 3 | BMC Biochemistry

Figure 3

From: Possibilities and pitfalls in quantifying the extent of cysteine sulfenic acid modification of specific proteins within complex biofluids

Figure 3

Relative percent abundance (RPA) of cysteine sulfenic acid on albumin (solid markers) and TTR (hollow markers) as reported by increasing concentrations of TNB. Plasma samples were prepared by oxidation with 4 mM hydrogen peroxide for 2 minutes at 37°C, followed by quenching with catalase, addition of TNB, immediate removal of TNB via gel filtration spin columns, 1000-fold dilution of plasma, and analysis by ESI-MS.

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BMC Biochemistry

ISSN: 1471-2091

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