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Figure 2 | BMC Biochemistry

Figure 2

From: Flavonoids activate pregnane × receptor-mediated CYP3A4 gene expression by inhibiting cyclin-dependent kinases in HepG2 liver carcinoma cells

Figure 2

Chrysin, apigenin, and luteolin are not potent PXR binders; p35 is expressed in HepG2. (A) Chrysin, apigenin, and luteolin are not potent PXR binders. SR12813, a potent human PXR agonist, was used as a positive control. Relative binding (%) was determined as described in Methods. Compounds were tested in triplicate. The bars denote the standard deviation. (B) p35 is expressed in HepG2 (lane 4). HEK 293T (293T) cells transfected with a p35 expression construct (293T/p35) (8 × 105 cells in 6-well plates transfected with 1 μg of pCMV-mycP35 using Fugene 6) was used as a positive control for p35 expression (lane 1). 293T was used as a negative control (lane 2). IMR-32, a neuroblastoma cell line that expresses p35 endogenously, was used as an additional positive control (lane 3). Total amount of lysate (μg) used is indicated above the samples. Since only 5 μg of total protein was used in lane 1, no actin band was detected.

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BMC Biochemistry

ISSN: 1471-2091

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